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Large-Grain and Semidwarf Isogenic Rice Koshihikari Integrated with GW2 and sd1

Author

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  • Motonori Tomita

    (Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka City 422-8529, Shizuoka, Japan)

  • Hideumi Ebata

    (Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka City 422-8529, Shizuoka, Japan)

  • Kohei Nakayama

    (Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka City 422-8529, Shizuoka, Japan)

Abstract

Intending to contribute to sustainable agriculture by “New Green Revolution,” we developed a large-grain/semidwarf isogenic line “Koshihikari sd1GW2” that incorporates both the large-grain gene GW2 and semidwarf gene sd1 . GW2 homozygous B 3 F 2 plant with the genomic background of Koshihikari was backcrossed twice with “Koshihikari sd1.” Koshihikari sd1GW2 fixed in BC 5 F 3 was found to be 12.6 cm shorter than Koshihikari. Whole-genome sequencing proved one deletion in GW2 at 8,147,416 bp on chromosome 2 and the SNPs in sd1 at 38,267,510 bp on chromosome 1. The size of the DNA fragments integrated with each gene was determined as the distance between both ends of SNP clusters. Through the backcrossing from BC 4 to BC 5 , the DNA fragment integrated with GW2 decreased by 148,139 bp. The thousand-grain weight of Koshihikari sd1GW2 (27.8 g) was 18% greater than that of Koshihikari (23.6 g), and the grain yield of Koshihikari sd1GW2 (42.6 kg/a) was 0.5% higher than that of Koshihikari (42.4 kg/a). Our results suggested that Koshihikari sd1GW2 will be less susceptible to lodging by typhoons, cyclones, and heavy rainfall, ordinarily a concern in heavier panicle weight cultivars. We successfully integrated GW2 with sd1 for the first time, specifically in the genome of the leading, globally produced Japonica cultivar Koshihikari.

Suggested Citation

  • Motonori Tomita & Hideumi Ebata & Kohei Nakayama, 2022. "Large-Grain and Semidwarf Isogenic Rice Koshihikari Integrated with GW2 and sd1," Sustainability, MDPI, vol. 14(17), pages 1-15, September.
    • Handle: RePEc:gam:jsusta:v:14:y:2022:i:17:p:11075-:d:907115
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    References listed on IDEAS

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    1. A. Sasaki & M. Ashikari & M. Ueguchi-Tanaka & H. Itoh & A. Nishimura & D. Swapan & K. Ishiyama & T. Saito & M. Kobayashi & G. S. Khush & H. Kitano & M. Matsuoka, 2002. "A mutant gibberellin-synthesis gene in rice," Nature, Nature, vol. 416(6882), pages 701-702, April.
    2. Youlin Peng & Yungao Hu & Qian Qian & Deyong Ren, 2021. "Progress and Prospect of Breeding Utilization of Green Revolution Gene SD 1 in Rice," Agriculture, MDPI, vol. 11(7), pages 1-11, June.
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