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Evaluation of Reference Genes for Gene Expression Analysis in Eichhornia crassipes

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  • Jing Xu

    (Key Laboratory for Ecological Restoration and High Quality Development of Qinling Mountains in the Upper and Middle Reaches of Yellow River, School of Biological and Environmental Engineering, Academy of Eco Xi’an, Xi’an University, Xi’an 710065, China)

  • Jing Li

    (State Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, China)

  • Tianpeng Gao

    (Key Laboratory for Ecological Restoration and High Quality Development of Qinling Mountains in the Upper and Middle Reaches of Yellow River, School of Biological and Environmental Engineering, Academy of Eco Xi’an, Xi’an University, Xi’an 710065, China)

Abstract

Eichhornia crassipes is a notorious invasive aquatic weed, causing enormous ecological and economic losses worldwide. However, it has great potential in agriculture, industry, medical care, and other areas. While being such an important plant, it is poorly understood from the molecular perspective. Aiming to select suitable reference genes for gene expression quantification in E. crassipes , this study favors future research at the molecular level. In this work, 12 candidate reference genes were selected. Their expression stability in samples of different tissues, samples treated with various hormones, samples supplied with different levels of phosphorus (P), and pooled samples, were analyzed using GeNorm, NormFinder, BestKeeper, and RefFinder. Meanwhile, the optimal number of reference genes was calculated by GeNorm. The results showed that eIF and ElF1a were the two most stable reference genes in all samples and in tissue samples. In response to hormone treatments, Actin and eIF are the best choices of internal controls. In the case of P treatments, TUA and H2A are recommended to be used as reference genes. Overall, results from this work suggest different reference genes should be applied in qRT-PCR on E. crassipes, according to the specific experimental setup.

Suggested Citation

  • Jing Xu & Jing Li & Tianpeng Gao, 2022. "Evaluation of Reference Genes for Gene Expression Analysis in Eichhornia crassipes," Sustainability, MDPI, vol. 14(17), pages 1-11, September.
    • Handle: RePEc:gam:jsusta:v:14:y:2022:i:17:p:11071-:d:907119
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    References listed on IDEAS

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    1. Obianuju P. Ilo & Mulala D. Simatele & S’phumelele L. Nkomo & Ntandoyenkosi M. Mkhize & Nagendra G. Prabhu, 2020. "The Benefits of Water Hyacinth ( Eichhornia crassipes ) for Southern Africa: A Review," Sustainability, MDPI, vol. 12(21), pages 1-20, November.
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