IDEAS home Printed from https://ideas.repec.org/a/gam/jsusta/v13y2021i8p4111-d531577.html
   My bibliography  Save this article

Analysis of Microbial Communities in Aged Refuse Based on 16S Sequencing

Author

Listed:
  • Fen Hou

    (Department of Land Resource Management, School of Public Administration, Shanxi University of Finance and Economics, Taiyuan 030012, China)

  • Junjie Du

    (College of Life Science, Shanxi Normal University, Linfen 710062, China)

  • Ye Yuan

    (Department of Land Resource Management, School of Public Administration, Shanxi University of Finance and Economics, Taiyuan 030012, China)

  • Xihui Wu

    (Department of Land Resource Management, School of Public Administration, Shanxi University of Finance and Economics, Taiyuan 030012, China)

  • Sai Zhao

    (Department of Land Resource Management, School of Public Administration, Shanxi University of Finance and Economics, Taiyuan 030012, China)

Abstract

Aged refuse is widely considered to have certain soil fertility. 16S rRNA amplicon sequencing is used to investigate the microbial community of aged refuse. The aged refuse is found to contain higher soil fertility elements (total nitrogen, total phosphorus, total potassium, etc.) and higher concentrations of heavy metals (Pb, Cd, Zn, and Hg). Taxonomy based on operational taxonomic units (OTUs) shows that Actinobacteria , Proteobacteria , Chloroflexi , Acidobacteria , and Gemmatimonadetes are the main bacterial phyla in the two soils and there is a palpable difference in the microbial community composition between the two groups of samples. The genera Paramaledivibacter , Limnochorda , Marinobacter , Pseudaminobacter , Kocuria , Bdellovibrio , Halomonas , Gillisia , and Membranicola are enriched in the aged refuse. Functional predictive analysis shows that both the control soil and aged refuse have a high abundance of “carbohydrate metabolism” and “amino acid metabolism”, and show differences in the abundance of several metabolism pathways, such as “xenobiotics biodegradation and metabolism” and “lipid metabolism”. Aged refuse and undisturbed soil show significant differences in alpha diversity and microbial community composition. Multiple environmental factors (Hg, TN, Cr, Cd, etc.) significantly impact microorganisms’ abundance ( Marinobacter , Halomonas , Blastococcus , etc.). Our study provides valuable knowledge for the ecological restoration of closed landfills.

Suggested Citation

  • Fen Hou & Junjie Du & Ye Yuan & Xihui Wu & Sai Zhao, 2021. "Analysis of Microbial Communities in Aged Refuse Based on 16S Sequencing," Sustainability, MDPI, vol. 13(8), pages 1-13, April.
    • Handle: RePEc:gam:jsusta:v:13:y:2021:i:8:p:4111-:d:531577
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2071-1050/13/8/4111/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2071-1050/13/8/4111/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Isabella Pecorini & Renato Iannelli, 2020. "Characterization of Excavated Waste of Different Ages in View of Multiple Resource Recovery in Landfill Mining," Sustainability, MDPI, vol. 12(5), pages 1-20, February.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Elena Rossi & Isabella Pecorini & Giovanni Ferrara & Renato Iannelli, 2022. "Dry Anaerobic Digestion of the Organic Fraction of Municipal Solid Waste: Biogas Production Optimization by Reducing Ammonia Inhibition," Energies, MDPI, vol. 15(15), pages 1-17, July.
    2. Inna Pitak & Gintaras Denafas & Arūnas Baltušnikas & Marius Praspaliauskas & Stasė-Irena Lukošiūtė, 2023. "Proposal for Implementation of Extraction Mechanism of Raw Materials during Landfill Mining and Its Application in Alternative Fuel Production," Sustainability, MDPI, vol. 15(5), pages 1-22, March.
    3. Isabella Pecorini & Elena Rossi & Renato Iannelli, 2020. "Bromatological, Proximate and Ultimate Analysis of OFMSW for Different Seasons and Collection Systems," Sustainability, MDPI, vol. 12(7), pages 1-20, March.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jsusta:v:13:y:2021:i:8:p:4111-:d:531577. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.