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Pro197Thr Substitution in Ahas Gene Causing Resistance to Pyroxsulam Herbicide in Rigid Ryegrass ( Lolium Rigidum Gaud.)

Author

Listed:
  • Barbara Kutasy

    (Campus Keszthely, Department of Plant Physiology and Plant Ecology, Hungarian University of Agriculture and Life Sciences Georgikon, 7 Festetics Str., 8360 Keszthely, Hungary)

  • Zsolt Takács

    (Department of Agricultural Mechanization, University of Pannonia, 16 Deák F. Str., 8360 Keszthely, Hungary)

  • Judit Kovács

    (Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Vienna Biocenter, Dr. Bohr-Gasse 3, 1030 Vienna, Austria)

  • Verëlindë Bogaj

    (Institute of Plant Protection, University of Pannonia, 16 Deák F. Str., 8360 Keszthely, Hungary)

  • Syafiq A. Razak

    (Institute of Plant Protection, University of Pannonia, 16 Deák F. Str., 8360 Keszthely, Hungary)

  • Géza Hegedűs

    (Educomat Ltd., 12/A Iskola Str., 8360 Keszthely, Hungary)

  • Kincső Decsi

    (Campus Keszthely, Department of Plant Physiology and Plant Ecology, Hungarian University of Agriculture and Life Sciences Georgikon, 7 Festetics Str., 8360 Keszthely, Hungary)

  • Kinga Székvári

    (Festetics Doctoral School, Hungarian University of Agriculture and Life Sciences, 7 Festetics Str., 8360 Keszthely, Hungary)

  • Eszter Virág

    (Educomat Ltd., 12/A Iskola Str., 8360 Keszthely, Hungary
    Department of Molecular Biotechnology and Microbiology, Institute of Biotechnology, Faculty of Science and Technology, University of Debrecen, 1 Egyetem Square, 4032 Debrecen, Hungary)

Abstract

Lolium rigidum Gaud. is a cross-pollinated species characterized by high genetic diversity and it was detected as one of the most herbicide resistance-prone weeds, globally. Acetohydroxyacid synthase (AHAS) resistant populations cause significant problems in cereal production; therefore, monitoring the development of AHAS resistance is widely recommended. Using next-generation sequencing (NGS), a de novo transcriptome sequencing dataset was presented to identify the complete open reading frame (ORF) of AHAS enzyme in L. rigidum and design markers to amplify fragments consisting of all of the eight resistance-conferring amino acid mutation sites. Pro197Thr, Pro197Ala, Pro197Ser, Pro197Gln, and Trp574Leu amino acid substitutions have been observed in samples. Although the Pro197Thr amino acid substitution was already described in SU and IMI resistant populations, this is the first report to reveal that the Pro197Thr in AHAS enzyme confers a high level of resistance (ED 50 3.569) to pyroxsulam herbicide (Triazolopyrimidine).

Suggested Citation

  • Barbara Kutasy & Zsolt Takács & Judit Kovács & Verëlindë Bogaj & Syafiq A. Razak & Géza Hegedűs & Kincső Decsi & Kinga Székvári & Eszter Virág, 2021. "Pro197Thr Substitution in Ahas Gene Causing Resistance to Pyroxsulam Herbicide in Rigid Ryegrass ( Lolium Rigidum Gaud.)," Sustainability, MDPI, vol. 13(12), pages 1-10, June.
    • Handle: RePEc:gam:jsusta:v:13:y:2021:i:12:p:6648-:d:572795
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    Cited by:

    1. Balázs Varga, 2023. "Plant Breeding Supporting the Sustainable Field Crop Production," Sustainability, MDPI, vol. 15(5), pages 1-5, February.

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