Pb(II) Bio-Removal, Viability, and Population Distribution of an Industrial Microbial Consortium: The Effect of Pb(II) and Nutrient Concentrations

This study presents the effect of aqueous Pb(II) and nutrient concentrations on the Pb(II)-removal, biomass viability, active species identities, and population distribution of an industrial Pb(II) resistant microbial consortium. The studied consortium has previously shown to be highly effective at precipitating Pb(II) from solution. At all conditions tested (80 and 500 ppm Pb(II), and varying nutrients conditions) it was found that circa 50% of Pb(II) was removed within the first 3 h, with the absence of any visual changes, followed by a slower rate of Pb(II) removal accompanied by the formation of a dark precipitate. The Pb(II) removal was found to be independent of microbial growth, while growth was observed dependent on the concentration of Pb(II), nutrients, and nitrates in the system. SEM analysis indicated viable bacilli embedded in precipitate. These findings indicate that precipitation occurs on the surface of the biomass as opposed to an internal excretion mechanism. BLAST (Basic Local Alignment Search Tool) results indicated Klebsiella pneumoniae as the active species responsible for Pb(II) bioprecipitation for both the 80 and 500 ppm isolated colonies, while a diverse population distribution of organisms was observed for the streak plate analyses. A quicker microbial generation rate was observed than what was expected for Klebsiella pneumoniae , indicating that the overall consortial population contributed to the growth rates observed. This study provided insights into the factors affecting Pb(II) bio-removal and bioprecipitation by the investigated industrially obtained consortium, thereby providing invaluable knowledge required for industrial application.