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Identification of the Red-Necked Longhorn Beetle Aromia bungii (Faldermann, 1835) (Coleoptera: Cerambycidae) with Real-Time PCR on Frass

Author

Listed:
  • Domenico Rizzo

    (Laboratory of Phytopathological Diagnostics and Molecular Biology, Plant Protection Service of Tuscany, 51100 Pistoia, Italy)

  • Andrea Taddei

    (ANSES Plant Health Laboratory, Entomology and Invasive Plants Unit, 34988 Montferrier-sur-Lez CEDEX, France)

  • Daniele Da Lio

    (Department of Agricultural, Food and Agro-Environmental Sciences, University of Pisa, 56124 Pisa, Italy)

  • Francesco Nugnes

    (National Research Council (CNR), Institute for Sustainable Plant Protection, 80055 Portici, Italy)

  • Eleonora Barra

    (Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy)

  • Luciana Stefani

    (Laboratory of Phytopathological Diagnostics and Molecular Biology, Plant Protection Service of Tuscany, 51100 Pistoia, Italy)

  • Linda Bartolini

    (Laboratory of Phytopathological Diagnostics and Molecular Biology, Plant Protection Service of Tuscany, 51100 Pistoia, Italy)

  • Raffaele V. Griffo

    (Plant Protection Service of Campania, Centro Direzionale, 80124 Napoli, Italy)

  • Paola Spigno

    (Phytopathological Laboratory, Campania Region, 80141 Napoli, Italy)

  • Lucia Cozzolino

    (Phytopathological Laboratory, Campania Region, 80141 Napoli, Italy)

  • Elisabetta Rossi

    (Department of Agricultural, Food and Agro-Environmental Sciences, University of Pisa, 56124 Pisa, Italy)

  • Antonio P. Garonna

    (Department of Agricultural Sciences, University of Naples Federico II, 80055 Portici, Italy)

Abstract

Aromia bungii (Faldermann, 1835) (Coleoptera: Cerambycidae), the red-necked longhorn beetle is native to eastern Asia, where it is an important wood-borer of fruit and ornamental species of the genus Prunus . A. bungii is a quarantine pest in the European Union, following its accidental introduction and establishment in Germany and Italy, and is currently included in the list of priority pests. To confirm its infestations in outbreak areas, adult or larval specimens are needed to perform morphological or molecular analyses. The presence of A. bungii larvae inside the attacked trees makes the collection of specimens particularly difficult. Thus, we present two diagnostic protocols based on frass analysis with real-time PCR (probe and SYBR Green). The results obtained show that a non-invasive approach for detecting the presence of this harmful invasive pest can be a reliable and accurate alternative diagnostic tool in phytosanitary surveys, as well as to outline a sustainable pest management strategy.

Suggested Citation

  • Domenico Rizzo & Andrea Taddei & Daniele Da Lio & Francesco Nugnes & Eleonora Barra & Luciana Stefani & Linda Bartolini & Raffaele V. Griffo & Paola Spigno & Lucia Cozzolino & Elisabetta Rossi & Anton, 2020. "Identification of the Red-Necked Longhorn Beetle Aromia bungii (Faldermann, 1835) (Coleoptera: Cerambycidae) with Real-Time PCR on Frass," Sustainability, MDPI, vol. 12(15), pages 1-12, July.
    • Handle: RePEc:gam:jsusta:v:12:y:2020:i:15:p:6041-:d:390797
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