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Different Length (DL) qPCR for Quantification of Cell Killing by UV-induced DNA Damage

Author

Listed:
  • Knut Rudi

    (Hedmark University College, Lærerskolealleen 1, 2418 Elverum, Norway
    NOFIMA Mat, Ås, Norway)

  • Irina Hagen

    (Hedmark University College, Lærerskolealleen 1, 2418 Elverum, Norway)

  • Bente Carina Johnsrud

    (Hedmark University College, Lærerskolealleen 1, 2418 Elverum, Norway)

  • Guro Skjefstad

    (Hedmark University College, Lærerskolealleen 1, 2418 Elverum, Norway)

  • Ingun Tryland

    (NIVA Norwegian Water Research Institute, Oslo, Norway)

Abstract

We describe the different length (DL) qPCR method for quantification of UV induced DNA damage in cell killing. The principle of DL qPCR is that DNA damage inhibits PCR. Applications with different lengths can therefore be used to detect different levels of UV-induced DNA damage. The assay was evaluated on three strains of Escherichia coli exposed to varying levels of ultraviolet (UV) radiation. We show that DL qPCR sensitivity and reproducibility are within the range of practical application to detect the effect of UV cell killing.

Suggested Citation

  • Knut Rudi & Irina Hagen & Bente Carina Johnsrud & Guro Skjefstad & Ingun Tryland, 2010. "Different Length (DL) qPCR for Quantification of Cell Killing by UV-induced DNA Damage," IJERPH, MDPI, vol. 7(9), pages 1-6, August.
    • Handle: RePEc:gam:jijerp:v:7:y:2010:i:9:p:3376-3381:d:9456
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