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Induction of Cell Death, DNA Strand Breaks, and Cell Cycle Arrest in DU145 Human Prostate Carcinoma Cell Line by Benzo[a]pyrene and Benzo[a]pyrene-7,8-diol-9,10-epoxide

Author

Listed:
  • Onyinye Nwagbara

    (Environmental Sciences Institute, Florida A&M University, Tallahassee, FL 32307, USA)

  • Selina F. Darling-Reed

    (College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, FL 32307, USA)

  • Alicia Tucker

    (College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, FL 32307, USA)

  • Cynthia Harris

    (Institute of Public Health, Florida A&M University, Tallahassee, FL 32307, USA)

  • Michael Abazinge

    (Environmental Sciences Institute, Florida A&M University, Tallahassee, FL 32307, USA)

  • Ronald D. Thomas

    (College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, FL 32307, USA)

  • Richard D. Gragg

    (Environmental Sciences Institute, Florida A&M University, Tallahassee, FL 32307, USA)

Abstract

Benzo[a]pyrene (B[a]P), a polycyclic aromatic hydrocarbon, is a major environmental pollutant. In this study, the effects of this carcinogen/mutagen and one of its metabolites, benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE), on human prostate carcinoma cell line DU145, were examined. Cell viability, DNA damage, and cell cycle progression were evaluated as toxic end-points. We have shown that B[a]P and BPDE inhibited cell viability following 48 hr of exposure. Furthermore, comet assay analyses revealed that both B[a]P and BPDE induced DNA strand breaks in a concentration -dependent fashion. Flow cytometric analyses showed that about 70 % of DU145 cells were arrested by B[a]P at the G1 phase, while about 76% were arrested at G1 phase by BPDE. These data reveal that B[a]P and BPDE are cytotoxic and genotoxic to DU145 prostate cancer cells.

Suggested Citation

  • Onyinye Nwagbara & Selina F. Darling-Reed & Alicia Tucker & Cynthia Harris & Michael Abazinge & Ronald D. Thomas & Richard D. Gragg, 2007. "Induction of Cell Death, DNA Strand Breaks, and Cell Cycle Arrest in DU145 Human Prostate Carcinoma Cell Line by Benzo[a]pyrene and Benzo[a]pyrene-7,8-diol-9,10-epoxide," IJERPH, MDPI, vol. 4(1), pages 1-5, March.
    • Handle: RePEc:gam:jijerp:v:4:y:2007:i:1:p:10-14:d:2309
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