IDEAS home Printed from https://ideas.repec.org/a/gam/jijerp/v2y2005i3p411-419d2757.html
   My bibliography  Save this article

Assessment of Cellular Responses to Oxidative Stress using MCF-7 Breast Cancer Cells, Black Seed ( N. Sativa L. ) Extracts and H 2 O 2

Author

Listed:
  • Ibrahim O. Farah

    (Department of Biology, Jackson State University, Jackson, MS 39217, USA)

Abstract

Black seed ( N. Sativa L ) is an oriental spice of the family Ranunculaceae that has long been rationally used as a natural medicine for treatment of many acute as well as chronic conditions including cardiovascular disease and immunological disorders. It has been used in the treatment of diabetes, hypertension, and dermatological conditions. There have been very few studies on the effects of N. Sativa as a chemoprevention of chronic diseases as well as in cancer prevention and/or therapy. Oxidative stress is a condition that underlies many acute as well as chronic conditions. The combination and role of oxidative stress and antioxidants in vivo is still a matter of conjecture. Our objective for the present study was to expose MCF-7 breast cancer cells in vitro (as a chronic disease example) to aqueous and alcohol extracts and in combination with H 2 O 2 as an oxidative stressor. Measurement of cell survival under various concentrations and mixtures was conducted using standard cell culture techniques, exposure protocols in 96 well plates and Fluorospectrosphotometry. Following cellular growth to 90% confluencey, exposure to water (WE) and ethanol (AE) extracts of N. sativa and H 2 O 2 was performed. Cell survival indices were calculated from percent survival using regression analysis. Results showed that the alcohol extract and its mixtures were able to influence the survival of MCF-7 cells (indices ranged from 357.15- 809.50 Bg/ml in descending potency for H 2 O 2 +AE to the mix of 3). In contrast, H 2 O 2 alone reduced effectively the survival of MCF-7 cells and the least effective combinations in descending potency were AE+H 2 O 2 , WE+H 2 O 2 , AE+WE, and WE+AE+H 2 O 2 . Mixtures other than AE+H 2 O 2 showed possible interactions and loss of potency. In conclusion, N. Sativa alone or in combination with oxidative stress was found to be effective ( in vitro ) in influencing the survival of MCF-7 breast cancer cells, unveiling promising opportunities in the field of cancer chemoprevention and/or treatment.

Suggested Citation

  • Ibrahim O. Farah, 2005. "Assessment of Cellular Responses to Oxidative Stress using MCF-7 Breast Cancer Cells, Black Seed ( N. Sativa L. ) Extracts and H 2 O 2," IJERPH, MDPI, vol. 2(3), pages 1-9, December.
    • Handle: RePEc:gam:jijerp:v:2:y:2005:i:3:p:411-419:d:2757
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1660-4601/2/3/411/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1660-4601/2/3/411/
    Download Restriction: no
    ---><---

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jijerp:v:2:y:2005:i:3:p:411-419:d:2757. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.