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Characterisation of Salmonella Enteritidis ST11 and ST1925 Associated with Human Intestinal and Extra-Intestinal Infections in Singapore

Author

Listed:
  • Kyaw Thu Aung

    (National Centre for Food Science, Singapore Food Agency, Singapore 718837, Singapore
    Environmental Health Institute, National Environment Agency, Singapore 138667, Singapore
    Nanyang Technological University Food Technology Centre (NAFTEC), Singapore 637459, Singapore
    School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637459, Singapore)

  • Wei Ching Khor

    (National Centre for Food Science, Singapore Food Agency, Singapore 718837, Singapore)

  • Kar Hui Ong

    (National Centre for Food Science, Singapore Food Agency, Singapore 718837, Singapore
    Environmental Health Institute, National Environment Agency, Singapore 138667, Singapore)

  • Wei Ling Tan

    (National Centre for Food Science, Singapore Food Agency, Singapore 718837, Singapore)

  • Zhi Ning Wong

    (National Centre for Food Science, Singapore Food Agency, Singapore 718837, Singapore)

  • Jia Quan Oh

    (National Centre for Food Science, Singapore Food Agency, Singapore 718837, Singapore
    Environmental Health Institute, National Environment Agency, Singapore 138667, Singapore)

  • Wai Kwan Wong

    (Animal & Veterinary Service, National Parks Board, Singapore 718827, Singapore)

  • Brian Zi Yan Tan

    (Animal & Veterinary Service, National Parks Board, Singapore 718827, Singapore)

  • Matthias Maiwald

    (Department of Pathology and Laboratory Medicine, KK Women’s and Children’s Hospital, Singapore 229899, Singapore
    Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117545, Singapore
    Duke-NUS Graduate Medical School, National University of Singapore, Singapore 169857, Singapore)

  • Nancy Wen Sim Tee

    (Department of Laboratory Medicine, National University Hospital, Singapore 119074, Singapore)

  • Timothy Barkham

    (Department of Laboratory Medicine, Tan Tock Seng Hospital, Singapore 308433, Singapore)

  • Tse Hsien Koh

    (Department of Pathology, Singapore General Hospital, Singapore 169856, Singapore)

  • Anders Dalsgaard

    (Nanyang Technological University Food Technology Centre (NAFTEC), Singapore 637459, Singapore
    School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637459, Singapore
    Department of Veterinary and Animal Sciences, University of Copenhagen, 1871 Frederiksberg, Denmark)

  • Swaine L. Chen

    (NUHS Infectious Diseases Translational Research Programme and Department of Medicine, Division of Infectious Diseases, Yong Loo Lin School of Medicine, National University of Singapore, 1E Kent Ridge Road, NUHS Tower Block, Singapore 119228, Singapore
    Laboratory of Bacterial Genomics, Genome Institute of Singapore, 60 Biopolis Street, Singapore 138672, Singapore)

  • Joergen Schlundt

    (Nanyang Technological University Food Technology Centre (NAFTEC), Singapore 637459, Singapore
    School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore 637459, Singapore)

  • Lee Ching Ng

    (Environmental Health Institute, National Environment Agency, Singapore 138667, Singapore
    School of Biological Sciences, Nanyang Technological University, Singapore 637551, Singapore)

Abstract

Salmonella Enteritidis is a major foodborne pathogen worldwide. In this study, a total of 276 S. enteritidis isolates, collected between 2016 and 2017 from human, food and farm/slaughterhouse samples, were studied to enhance the understanding of the epidemiology of human salmonellosis in Singapore. Results showed all 276 isolates belonged either to ST1925 (70.3%) or ST11 (29.7%), with ST11 being significantly more frequent in extra-intestinal isolates and chicken isolates. Food isolates, most of which were from poultry, showed the highest prevalence of resistance (33–37%) against beta-lactams or beta-lactams/beta-lactamase inhibitor combination (ampicillin, piperacillin and ampicillin/sulbactam). The analysis showed the detection of genes associated with resistance to aminoglycoside genes (99.6%), tetracycline (55.1%), and beta-lactams (14.9%) of all isolates. Nine types of plasmids were found in 266 isolates; the most common incompatibility group profiles were IncFIB(S)-IncFII(S)-IncX1 (72.2%) and IncFIB(S)-IncFII(S) (15.8%). Most plasmid harbouring isolates from chicken (63.6%, 14/22) and from human (73.8%, 175/237) shared the same plasmid profile (IncFIB(S)-IncFII(S)-IncX1). SNP analysis showed clustering of several isolates from poultry food products and human isolates, suggesting phylogenetic relatedness among these isolates. Lastly, this study provides important epidemiological insights on the application of phenotypic and next-generation sequencing (NGS) tools for improved food safety and public health surveillance and outbreak investigation of S. enteritidis .

Suggested Citation

  • Kyaw Thu Aung & Wei Ching Khor & Kar Hui Ong & Wei Ling Tan & Zhi Ning Wong & Jia Quan Oh & Wai Kwan Wong & Brian Zi Yan Tan & Matthias Maiwald & Nancy Wen Sim Tee & Timothy Barkham & Tse Hsien Koh & , 2022. "Characterisation of Salmonella Enteritidis ST11 and ST1925 Associated with Human Intestinal and Extra-Intestinal Infections in Singapore," IJERPH, MDPI, vol. 19(9), pages 1-14, May.
    • Handle: RePEc:gam:jijerp:v:19:y:2022:i:9:p:5671-:d:809955
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    References listed on IDEAS

    as
    1. Derniza Cozorici & Roxana-Alexandra Măciucă & Costel Stancu & Bianca-Maria Tihăuan & Robert Bogdan Uță & Cosmin Iulian Codrea & Răzvan Matache & Cristian-Emilian Pop & Robert Wolff & Sergiu Fendrihan, 2022. "Microbial Contamination and Survival Rate on Different Types of Banknotes," IJERPH, MDPI, vol. 19(7), pages 1-13, April.
    2. Kyaw Thu Aung & Hong Jun Chen & Man Ling Chau & Grace Yap & Xiao Fang Lim & Mahathir Humaidi & Cliff Chua & Gladys Yeo & Hooi Ming Yap & Jia Quan Oh & Vijitha Manogaran & Hapuarachchige Chanditha Hapu, 2019. "Salmonella in Retail Food and Wild Birds in Singapore—Prevalence, Antimicrobial Resistance, and Sequence Types," IJERPH, MDPI, vol. 16(21), pages 1-12, October.
    3. Kyaw Thu Aung & Wei Ching Khor & Sophie Octavia & Agnes Ye & Justina Leo & Pei Pei Chan & Georgina Lim & Wai Kwan Wong & Brian Zi Yan Tan & Joergen Schlundt & Anders Dalsgaard & Lee Ching Ng & Yueh Nu, 2020. "Distribution of Salmonella Serovars in Humans, Foods, Farm Animals and Environment, Companion and Wildlife Animals in Singapore," IJERPH, MDPI, vol. 17(16), pages 1-13, August.
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