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Keratinolytic Properties of Aspergillus clavatus Promising for Biodegradation

Author

Listed:
  • Svetlana Timorshina

    (Department of Microbiology, Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia)

  • Elizaveta Popova

    (Department of Microbiology, Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia)

  • Valeriana Kreyer

    (Department of Microbiology, Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia)

  • Nina Baranova

    (Department of Microbiology, Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia)

  • Alexander Osmolovskiy

    (Department of Microbiology, Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia)

Abstract

The high demand for keratinolytic enzymes and the modest presentation of fungal keratinase diversity studies in scientific sources cause a significant interest in identifying new fungal strains of keratinase producers, isolating new enzymes and studying their properties. Four out of the 32 cultures showed a promising target activity on protein-containing agar plates— Aspergillus amstelodami A6, A. clavatus VKPM F-1593, A. ochraceus 247, and Cladosporium sphaerospermum 1779. The highest values of keratinolytic activity were demonstrated by extracellular proteins synthesized by Aspergillus clavatus VKPM F-1593 cultivated under submerged conditions on a medium containing milled chicken feathers. The enzyme complex preparation was obtained by protein precipitation from the culture liquid with ammonium sulfate, subsequent dialysis, and lyophilization. The fraction of a pure enzyme with keratinolytic activity (pI 9.3) was isolated by separating the extracellular proteins of A. clavatus VKPM F-1593 via isoelectric focusing. The studied keratinase was an alkaline subtilisin-like non-glycosylated protease active over a wide pH range with optimum keratinolysis at pH 8 and 50 °C.

Suggested Citation

  • Svetlana Timorshina & Elizaveta Popova & Valeriana Kreyer & Nina Baranova & Alexander Osmolovskiy, 2022. "Keratinolytic Properties of Aspergillus clavatus Promising for Biodegradation," IJERPH, MDPI, vol. 19(21), pages 1-17, October.
    • Handle: RePEc:gam:jijerp:v:19:y:2022:i:21:p:13939-:d:954338
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    References listed on IDEAS

    as
    1. Huayi Chen & Shuang Gao & Yongtao Li & Hui-Juan Xu & Wenyan Li & Jinjin Wang & Yulong Zhang, 2022. "Valorization of Livestock Keratin Waste: Application in Agricultural Fields," IJERPH, MDPI, vol. 19(11), pages 1-18, May.
    2. Michał Możejko & Justyna Bohacz, 2022. "Optimization of Conditions for Feather Waste Biodegradation by Geophilic Trichophyton ajelloi Fungal Strains towards Further Agricultural Use," IJERPH, MDPI, vol. 19(17), pages 1-30, August.
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