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Fourier-Transform Infrared Spectroscopy as a Discriminatory Tool for Myotonic Dystrophy Type 1 Metabolism: A Pilot Study

Author

Listed:
  • Tiago Mateus

    (Department of Medical Sciences, Institute of Biomedicine (iBiMED), University of Aveiro, 3810-193 Aveiro, Portugal)

  • Idália Almeida

    (Department of Medical Sciences, Institute of Biomedicine (iBiMED), University of Aveiro, 3810-193 Aveiro, Portugal)

  • Adriana Costa

    (Department of Medical Sciences, Institute of Biomedicine (iBiMED), University of Aveiro, 3810-193 Aveiro, Portugal)

  • Diana Viegas

    (Department of Medical Sciences, Institute of Biomedicine (iBiMED), University of Aveiro, 3810-193 Aveiro, Portugal)

  • Sandra Magalhães

    (Department of Medical Sciences, Institute of Biomedicine (iBiMED), University of Aveiro, 3810-193 Aveiro, Portugal
    Department of Chemistry, Aveiro Institute of Materials (CICECO), University of Aveiro, 3810-193 Aveiro, Portugal)

  • Filipa Martins

    (Department of Medical Sciences, Institute of Biomedicine (iBiMED), University of Aveiro, 3810-193 Aveiro, Portugal)

  • Maria Teresa Herdeiro

    (Department of Medical Sciences, Institute of Biomedicine (iBiMED), University of Aveiro, 3810-193 Aveiro, Portugal)

  • Odete A. B. da Cruz e Silva

    (Department of Medical Sciences, Institute of Biomedicine (iBiMED), University of Aveiro, 3810-193 Aveiro, Portugal)

  • Carla Fraga

    (Neurology Department, Centro Hospitalar Tâmega e Sousa (CHTS), 4564-007 Penafiel, Portugal)

  • Ivânia Alves

    (Neurology Department, Centro Hospitalar Tâmega e Sousa (CHTS), 4564-007 Penafiel, Portugal)

  • Alexandra Nunes

    (Department of Medical Sciences, Institute of Biomedicine (iBiMED), University of Aveiro, 3810-193 Aveiro, Portugal)

  • Sandra Rebelo

    (Department of Medical Sciences, Institute of Biomedicine (iBiMED), University of Aveiro, 3810-193 Aveiro, Portugal)

Abstract

Myotonic dystrophy type 1 (DM1) is a hereditary disease characterized by progressive distal muscle weakness and myotonia. Patients with DM1 have abnormal lipid metabolism and a high propensity to develop a metabolic syndrome in comparison to the general population. It follows that metabolome evaluation in these patients is crucial and may contribute to a better characterization and discrimination between DM1 disease phenotypes and severities. Several experimental approaches are possible to carry out such an analysis; among them is Fourier-transform infrared spectroscopy (FTIR) which evaluates metabolic profiles by categorizing samples through their biochemical composition. In this study, FTIR spectra were acquired and analyzed using multivariate analysis (Principal Component Analysis) using skin DM1 patient-derived fibroblasts and controls. The results obtained showed a clear discrimination between both DM1-derived fibroblasts with different CTG repeat length and with the age of disease onset; this was evident given the distinct metabolic profiles obtained for the two groups. Discrimination could be attributed mainly to the altered lipid metabolism and proteins in the 1800–1500 cm −1 region. These results suggest that FTIR spectroscopy is a valuable tool to discriminate both DM1-derived fibroblasts with different CTG length and age of onset and to study the metabolomic profile of patients with DM1.

Suggested Citation

  • Tiago Mateus & Idália Almeida & Adriana Costa & Diana Viegas & Sandra Magalhães & Filipa Martins & Maria Teresa Herdeiro & Odete A. B. da Cruz e Silva & Carla Fraga & Ivânia Alves & Alexandra Nunes & , 2021. "Fourier-Transform Infrared Spectroscopy as a Discriminatory Tool for Myotonic Dystrophy Type 1 Metabolism: A Pilot Study," IJERPH, MDPI, vol. 18(7), pages 1-20, April.
    • Handle: RePEc:gam:jijerp:v:18:y:2021:i:7:p:3800-:d:530609
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    References listed on IDEAS

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    1. Tiago Mateus & Filipa Martins & Alexandra Nunes & Maria Teresa Herdeiro & Sandra Rebelo, 2021. "Metabolic Alterations in Myotonic Dystrophy Type 1 and Their Correlation with Lipin," IJERPH, MDPI, vol. 18(4), pages 1-22, February.
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