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A Fumigation-Based Surface Sterilization Approach for Plant Tissue Culture

Author

Listed:
  • Iyyakkannu Sivanesan

    (Department of Bioresources and Food Science, Konkuk University, 1, Hwayang-dong, Gwangjin-gu, Seoul 05029, Korea)

  • Manikandan Muthu

    (Laboratory of Neo Natural Farming, Chunnampet, Tamil Nadu 603 401, India)

  • Judy Gopal

    (Laboratory of Neo Natural Farming, Chunnampet, Tamil Nadu 603 401, India)

  • Shadma Tasneem

    (Department of Chemistry, Faculty of Science, Jazan University, Jazan P.O. Box 114, Jazan 45142, Saudi Arabia)

  • Doo-Hwan Kim

    (Department of Bioresources and Food Science, Konkuk University, 1, Hwayang-dong, Gwangjin-gu, Seoul 05029, Korea)

  • Jae-Wook Oh

    (Department of Stem Cell and Regenerative Biology, Konkuk University, Seoul 05029, Korea)

Abstract

Plant tissue culture has led to breakthroughs in understanding and applying the fundamental knowledge towards harnessing more from plants. Microbial contamination is one of the serious problems limiting the successful extrapolation of plant tissue culture practices. Sources of in vitro contamination include culture containers, media, explants, equipment, the environment of the culture room and transfer area, and operating personnel. The successful initiation of in vitro culture mostly depends on surface sterilization of explants because this is the primary source. Usually, surface sterilization is done using chemicals, or toxic nanomaterials, this is the first time such an approach has been demonstrated. Numerous surface microflora attached to plant surfaces grow faster than the cultured explants and release phytotoxic substances into the culture media, hindering positive outcomes. In the current work, for the first time, the applicability of turmeric and benzoin resin-based fumigation of explants is demonstrated. The results showed that fumigation methods for surface sterilization were promising and could lead to fifty and even 100% contamination-free plant tissue culture. Nanoparticulate carbon was identified in the turmeric and benzoin smoke and coined the key player in the surface sterilization effect. These studies open a whole new avenue for the use of fumigation-based methods for riddance of microbial contamination.

Suggested Citation

  • Iyyakkannu Sivanesan & Manikandan Muthu & Judy Gopal & Shadma Tasneem & Doo-Hwan Kim & Jae-Wook Oh, 2021. "A Fumigation-Based Surface Sterilization Approach for Plant Tissue Culture," IJERPH, MDPI, vol. 18(5), pages 1-11, February.
  • Handle: RePEc:gam:jijerp:v:18:y:2021:i:5:p:2282-:d:505788
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