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First Investigative Study of Azole-Resistant Aspergillus fumigatus in the Environment in Burkina Faso

Author

Listed:
  • Isidore W. Yerbanga

    (Ecoles Doctorales des Sciences de la Santé, Université Nazi Boni, Bobo-Dioulasso, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso
    Service de laboratoire, Centre Hospitalier Universitaire Régional de Ouahigouya, Ouahigouya, 01 BP 36 Ouahigouya 01, Burkina Faso)

  • Agustin Resendiz-Sharpe

    (Department of Microbiology, Immunology and Transplantation, KU Leuven, University Hospitals Leuven, Leuven, Herestraat 49-Box 1030, 3000 Leuven, Belgium)

  • Sanata Bamba

    (Ecoles Doctorales des Sciences de la Santé, Université Nazi Boni, Bobo-Dioulasso, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso
    Département des laboratoires, Centre Hospitalier Universitaire Sourô Sanou, Bobo-Dioulasso, 01 BP 676 Bobo-Dioulasso 01, Burkina Faso)

  • Katrien Lagrou

    (Department of Microbiology, Immunology and Transplantation, KU Leuven, University Hospitals Leuven, Leuven, Herestraat 49-Box 1030, 3000 Leuven, Belgium
    Department of Laboratory Medicine and National Reference Center for Mycosis, Excellence Center for Medical Mycology (ECMM), University Hospitals Leuven, Herestraat 49-Box 1030, 3000 Leuven, Belgium)

  • Seydou Nakanabo Diallo

    (Ecoles Doctorales des Sciences de la Santé, Université Nazi Boni, Bobo-Dioulasso, 01 BP 1091 Bobo-Dioulasso 01, Burkina Faso
    Programme de Recherche sur les Maladies Parasitaires et les Vecteurs, Centre Muraz, Institut National de Santé Publique, Bobo-Dioulasso, 01 BP 390 Bobo-Dioulasso 01, Burkina Faso)

  • Hector Rodriguez-Villalobos

    (Department of Microbiology, Cliniques Universitaires Saint-Luc–Université Catholique de Louvain, Brussels, Avenue Hippocrate 10, 1200 Bruxelles, Belgium)

  • Olivier Denis

    (Department of Microbiology, CHU Namur Site-Godinne, Université Catholique de Louvain, Brussels, Rue Dr Gaston Therasse 1, 5530 Yvoir, Belgium
    Ecole de Santé Publique, Université Libre de Bruxelles, Brussels, Route de Lennik 808, 1070 Anderlecht, Belgium)

  • Isabel Montesinos

    (Department of Microbiology, LHUB-ULB, Université Libre de Bruxelles, Brussels, Rue Haute 322, 1000 Bruxelles, Belgium)

Abstract

Azole-resistant Aspergillus fumigatus (ARAF) strains have been reported on all continents, however, limited data exist on these strains in Africa, while several factors, mainly environmental ones, suggest their presence on this continent. This study aimed to assess the environmental prevalence of ARAF strains in Burkina Faso, a country situated in the West African region where data on ARAF is non-existent. In total, 120 environmental samples (soil) were collected and analyzed. Samples were screened for resistance using three azole-containing agar plates; one without azole antifungal (growth control) and two supplemented with either itraconazole (4 mg/L) or voriconazole (2 mg/L). The EUCAST susceptibility testing method was used to confirm the azole-resistant phenotype of A. fumigatus sensu-stricto isolates. Mutations in the cyp51A gene were determined by sequencing. Of the 120 samples, 51 positive samples showed growth of A. fumigatus isolates on control medium. One ARAF (2%; 1/51) isolate was found amongst A. fumigatus positive samples and harbored the F46Y/M172V/E427K cyp51A mutations. No TR34/L98H or TR46/Y121F/T289A mutations were observed. Our study described the first A. fumigatus isolate resistant to an azole antifungal in Burkina Faso.

Suggested Citation

  • Isidore W. Yerbanga & Agustin Resendiz-Sharpe & Sanata Bamba & Katrien Lagrou & Seydou Nakanabo Diallo & Hector Rodriguez-Villalobos & Olivier Denis & Isabel Montesinos, 2021. "First Investigative Study of Azole-Resistant Aspergillus fumigatus in the Environment in Burkina Faso," IJERPH, MDPI, vol. 18(5), pages 1-6, February.
    • Handle: RePEc:gam:jijerp:v:18:y:2021:i:5:p:2250-:d:505302
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