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Phytoecdysteroids Do Not Have Anabolic Effects in Skeletal Muscle in Sedentary Aging Mice

Author

Listed:
  • Marcus M. Lawrence

    (Department of Health and Exercise Science, Appalachian State University, Boone, NC 28608, USA
    Human Performance Laboratory, North Carolina Research Campus, Kannapolis, NC 28081, USA
    Integrated Muscle Physiology Laboratory, Boone, NC 28607, USA
    Department of Kinesiology and Outdoor Recreation, Southern Utah University, Cedar City, UT 84720, USA)

  • Kevin A. Zwetsloot

    (Department of Health and Exercise Science, Appalachian State University, Boone, NC 28608, USA
    Integrated Muscle Physiology Laboratory, Boone, NC 28607, USA
    Department of Biology, Appalachian State University, Boone, NC 20608, USA)

  • Susan T. Arthur

    (Laboratory of Systems Physiology, Department of Kinesiology, University of North Carolina Charlotte, Charlotte, NC 28223, USA)

  • Chase A. Sherman

    (Department of Health and Exercise Science, Appalachian State University, Boone, NC 28608, USA
    Integrated Muscle Physiology Laboratory, Boone, NC 28607, USA)

  • Joshua R. Huot

    (Laboratory of Systems Physiology, Department of Kinesiology, University of North Carolina Charlotte, Charlotte, NC 28223, USA)

  • Vladimir Badmaev

    (American Medical Holdings Inc., Staten Island, NY 10314, USA)

  • Mary Grace

    (Plants for Human Health Institute, North Carolina Research Campus, North Carolina State University, Kannapolis, NC 28081, USA)

  • Mary Ann Lila

    (Plants for Human Health Institute, North Carolina Research Campus, North Carolina State University, Kannapolis, NC 28081, USA)

  • David C. Nieman

    (Human Performance Laboratory, North Carolina Research Campus, Kannapolis, NC 28081, USA
    Department of Biology, Appalachian State University, Boone, NC 20608, USA)

  • R. Andrew Shanely

    (Department of Health and Exercise Science, Appalachian State University, Boone, NC 28608, USA
    Human Performance Laboratory, North Carolina Research Campus, Kannapolis, NC 28081, USA
    Integrated Muscle Physiology Laboratory, Boone, NC 28607, USA)

Abstract

Skeletal muscle mass and strength are lost with aging. Phytoecdysteroids, in particular 20-hydroxyecdysone (20E), increase protein synthesis in C2C12 skeletal muscle cells and muscle strength in young rats. The objective of this study was to determine whether an extract from Ajuga turkestanica (ATE), enriched in phytoecdysteroids, and 20E affect skeletal muscle mass and fiber size, fiber type, activation of the PI3K–Akt signaling pathway, and the mRNA levels of MAFbx , MuRF-1 , and myostatin in sedentary aging mice. Aging male C57BL/6 mice (20 months old) received ATE, 20E, or vehicle (CT) once per day for 28 days or a single acute dose. Treatment did not alter body, muscle, or organ mass; fiber cross-sectional area; or fiber type in the triceps brachii or plantaris muscles. Likewise, protein synthesis signaling markers (i.e., phosphorylation of Akt Ser473 and p70S6k Thr389 ) measured after either 28 days or acutely were unchanged. Neither ATE nor 20E treatment for 28 days affected the mRNA levels of MAFbx , MuRF-1 , and myostatin . In conclusion, these data indicate that phytoecdysteroid treatment does not alter muscle mass or fiber type, nor does it activate protein synthesis signaling in the skeletal muscle of sedentary aging mice.

Suggested Citation

  • Marcus M. Lawrence & Kevin A. Zwetsloot & Susan T. Arthur & Chase A. Sherman & Joshua R. Huot & Vladimir Badmaev & Mary Grace & Mary Ann Lila & David C. Nieman & R. Andrew Shanely, 2021. "Phytoecdysteroids Do Not Have Anabolic Effects in Skeletal Muscle in Sedentary Aging Mice," IJERPH, MDPI, vol. 18(2), pages 1-14, January.
    • Handle: RePEc:gam:jijerp:v:18:y:2021:i:2:p:370-:d:475407
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