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Characterization of IL-2 Stimulation and TRPM7 Pharmacomodulation in NK Cell Cytotoxicity and Channel Co-Localization with PIP 2 in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome Patients

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  • Stanley Du Preez

    (National Centre for Neuroimmunology and Emerging Diseases, Menzies Health Institute, Griffith University, Gold Coast 4215, Australia
    Consortium Health International for Myalgic Encephalomyelitis, Menzies Health Institute Queensland, Griffith University, Gold Coast 4215, Australia
    School of Pharmacy and Medical Sciences, Griffith University, Gold Coast 4215, Australia
    School of Medicine and Dentistry, Griffith University, Gold Coast 4215, Australia)

  • Natalie Eaton-Fitch

    (National Centre for Neuroimmunology and Emerging Diseases, Menzies Health Institute, Griffith University, Gold Coast 4215, Australia
    Consortium Health International for Myalgic Encephalomyelitis, Menzies Health Institute Queensland, Griffith University, Gold Coast 4215, Australia
    School of Pharmacy and Medical Sciences, Griffith University, Gold Coast 4215, Australia)

  • Helene Cabanas

    (Consortium Health International for Myalgic Encephalomyelitis, Menzies Health Institute Queensland, Griffith University, Gold Coast 4215, Australia
    INSERM U944 and CNRS UMR 7212, Institut de Recherche Saint Louis, Hôpital Saint Louis, APHP, Université de Paris, 75010 Paris, France)

  • Donald Staines

    (National Centre for Neuroimmunology and Emerging Diseases, Menzies Health Institute, Griffith University, Gold Coast 4215, Australia
    Consortium Health International for Myalgic Encephalomyelitis, Menzies Health Institute Queensland, Griffith University, Gold Coast 4215, Australia)

  • Sonya Marshall-Gradisnik

    (National Centre for Neuroimmunology and Emerging Diseases, Menzies Health Institute, Griffith University, Gold Coast 4215, Australia
    Consortium Health International for Myalgic Encephalomyelitis, Menzies Health Institute Queensland, Griffith University, Gold Coast 4215, Australia)

Abstract

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a complex multisystemic disorder responsible for significant disability. Although a unifying etiology for ME/CFS is uncertain, impaired natural killer (NK) cell cytotoxicity represents a consistent and measurable feature of this disorder. Research utilizing patient-derived NK cells has implicated dysregulated calcium (Ca 2+ ) signaling, dysfunction of the phosphatidylinositol-4,5-bisphosphate (PIP 2 )-dependent cation channel, transient receptor potential melastatin (TRPM) 3, as well as altered surface expression patterns of TRPM3 and TRPM2 in the pathophysiology of ME/CFS. TRPM7 is a related channel that is modulated by PIP 2 and participates in Ca 2+ signaling. Though TRPM7 is expressed on NK cells, the role of TRPM7 with IL-2 and intracellular signaling mechanisms in the NK cells of ME/CFS patients is unknown. This study examined the effect of IL-2 stimulation and TRPM7 pharmacomodulation on NK cell cytotoxicity using flow cytometric assays as well as co-localization of TRPM7 with PIP 2 and cortical actin using confocal microscopy in 17 ME/CFS patients and 17 age- and sex-matched healthy controls. The outcomes of this investigation are preliminary and indicate that crosstalk between IL-2 and TRMP7 exists. A larger sample size to confirm these findings and characterization of TRPM7 in ME/CFS using other experimental modalities are warranted.

Suggested Citation

  • Stanley Du Preez & Natalie Eaton-Fitch & Helene Cabanas & Donald Staines & Sonya Marshall-Gradisnik, 2021. "Characterization of IL-2 Stimulation and TRPM7 Pharmacomodulation in NK Cell Cytotoxicity and Channel Co-Localization with PIP 2 in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome Patients," IJERPH, MDPI, vol. 18(22), pages 1-18, November.
  • Handle: RePEc:gam:jijerp:v:18:y:2021:i:22:p:11879-:d:677707
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    References listed on IDEAS

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    1. Monica J. S. Nadler & Meredith C. Hermosura & Kazunori Inabe & Anne-Laure Perraud & Qiqin Zhu & Alexander J. Stokes & Tomohiro Kurosaki & Jean-Pierre Kinet & Reinhold Penner & Andrew M. Scharenberg & , 2001. "LTRPC7 is a Mg·ATP-regulated divalent cation channel required for cell viability," Nature, Nature, vol. 411(6837), pages 590-595, May.
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