IDEAS home Printed from https://ideas.repec.org/a/gam/jijerp/v18y2021i12p6269-d572202.html
   My bibliography  Save this article

Assessment of Cytogenetic Damage and Cholinesterases’ Activity in Workers Occupationally Exposed to Pesticides in Zamora-Jacona, Michoacan, Mexico

Author

Listed:
  • Rafael Valencia-Quintana

    (Laboratorio “Rafael Villalobos-Pietrini” de Toxicología Genómica y Química Ambiental, Facultad de Agrobiología, Universidad Autónoma de Tlaxcala, CA Genética y Ambiente UATLX-CA 223, Red Temática de Toxicología de Plaguicidas, Tlaxcala 90120, Mexico)

  • Rosa María López-Durán

    (Laboratorio de Biomembranas, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, Ciudad de México 09340, Mexico)

  • Mirta Milić

    (Mutagenesis Unit, Institute for Medical Research and Occupational Health, Ksaverska Cesta 2, 10000 Zagreb, Croatia)

  • Stefano Bonassi

    (Department of Human Sciences and Quality of Life Promotion, San Rafaele University, 00166 Rome, Italy
    Unit of Clinical and Molecular Epidemiology, IRCCS San Rafaele Pisana, 00166 Rome, Italy)

  • Ma. Antonieta Ochoa-Ocaña

    (Unidad Académica de Estudios Regionales, Coordinación de Humanidades, UNAM, Jiquilpan 59510, Mexico)

  • Mayrut Osdely Uriostegui-Acosta

    (Escuela Superior de Ciencias Naturales, Universidad Autónoma de Guerrero Chilpancingo 39105, Mexico)

  • Guillermo Alejandro Pérez-Flores

    (Laboratorio “Rafael Villalobos-Pietrini” de Toxicología Genómica y Química Ambiental, Facultad de Agrobiología, Universidad Autónoma de Tlaxcala, CA Genética y Ambiente UATLX-CA 223, Red Temática de Toxicología de Plaguicidas, Tlaxcala 90120, Mexico)

  • José Luis Gómez-Olivares

    (Laboratorio de Biomembranas, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, Ciudad de México 09340, Mexico)

  • Juana Sánchez-Alarcón

    (Laboratorio “Rafael Villalobos-Pietrini” de Toxicología Genómica y Química Ambiental, Facultad de Agrobiología, Universidad Autónoma de Tlaxcala, CA Genética y Ambiente UATLX-CA 223, Red Temática de Toxicología de Plaguicidas, Tlaxcala 90120, Mexico)

Abstract

Pesticides have been considered as potential chemical mutagens; however, little is known about toxic and genotoxic effects during pesticide application in Zamora-Jacona, Michoacan State in Mexico. This study sought to determine DNA damage and cholinesterase activities inhibitions in 54 agricultural workers exposed to complex mixtures of pesticides vs. control group (26 individuals) using Comet assay in peripheral whole blood, micronucleus (MN) test in oral mucosa cells, Cytokinesis-blocked MN assay in lymphocytes (L-CBMNcyt) and measuring AChE and BChE activities in whole blood and plasma samples, respectively. Exposed subjects demonstrated significantly elevated levels of primary (Comet assay: tail intensity, tail length, tail moment, Olive tail moment) and permanent DNA damage (MN assay: in blood/buccal cells; frequencies of nuclear buds, binucleated cells, cells with condensed chromatin, karyorrhexis, pyknosis, and karyolysis). However, inhibition of cholinesterase activities (AChE and BChE) was not observed in the workers. Confounding factors including sex, age, BMI, working exposure period, protection level, smoking habit (cigarettes per day units), alcohol consumption (weekly), medication, were considered in the analysis. These combined techniques demonstrated usefulness in the health hazards risks pesticide exposure assessment and suggested the need for periodic monitoring together with the education and the training of occupational workers for the safe application of potentially harmful pesticides.

Suggested Citation

  • Rafael Valencia-Quintana & Rosa María López-Durán & Mirta Milić & Stefano Bonassi & Ma. Antonieta Ochoa-Ocaña & Mayrut Osdely Uriostegui-Acosta & Guillermo Alejandro Pérez-Flores & José Luis Gómez-Oli, 2021. "Assessment of Cytogenetic Damage and Cholinesterases’ Activity in Workers Occupationally Exposed to Pesticides in Zamora-Jacona, Michoacan, Mexico," IJERPH, MDPI, vol. 18(12), pages 1-17, June.
    • Handle: RePEc:gam:jijerp:v:18:y:2021:i:12:p:6269-:d:572202
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1660-4601/18/12/6269/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1660-4601/18/12/6269/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Qinghua Shi & Randall W. King, 2005. "Chromosome nondisjunction yields tetraploid rather than aneuploid cells in human cell lines," Nature, Nature, vol. 437(7061), pages 1038-1042, October.
    2. América I. Zamora-Torres & Estefanía Riveros-Figueroa, 2016. "Estudio de la región Michoacana de Zamora como polo de competitividad internacional agrícola," Revista Clio América, Universidad del Magdalena, vol. 10(20), pages 139-159, December.
    3. Ana Flavia Marcelino & Catia Cappelli Wachtel & Nédia de Castilhos Ghisi, 2019. "Are Our Farm Workers in Danger? Genetic Damage in Farmers Exposed to Pesticides," IJERPH, MDPI, vol. 16(3), pages 1-11, January.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Monica Lopes-Ferreira & Adolfo Luis Almeida Maleski & Leticia Balan-Lima & Jefferson Thiago Gonçalves Bernardo & Lucas Marques Hipolito & Ana Carolina Seni-Silva & Joao Batista-Filho & Maria Alice Pim, 2022. "Impact of Pesticides on Human Health in the Last Six Years in Brazil," IJERPH, MDPI, vol. 19(6), pages 1-19, March.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jijerp:v:18:y:2021:i:12:p:6269-:d:572202. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.