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Serological and Molecular Detection of Bovine Brucellosis at Institutional Livestock Farms in Punjab, Pakistan

Author

Listed:
  • Tariq Jamil

    (Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, 07743 Jena, Germany
    Institute of Microbiology and Epizootics, Freie Universität Berlin, 14163 Berlin, Germany)

  • Falk Melzer

    (Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, 07743 Jena, Germany)

  • Muhammad Saqib

    (Department of Clinical Medicine and Surgery, Faculty of Veterinary Science, University of Agriculture, Faisalabad 38000, Pakistan)

  • Asim Shahzad

    (Department of Pathology, Faculty of Veterinary Science, University of Agriculture, Faisalabad 38000, Pakistan)

  • Khushal Khan Kasi

    (Disease Investigation Laboratory, Livestock and Dairy Development Department, Government of Baluchistan, Quetta 87300, Pakistan
    Institute of Epidemiology, Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, Germany)

  • Muhammad Hammad Hussain

    (1 Vance Street, 2565 Bardia, New South Wales, Australia)

  • Imaad Rashid

    (Department of Clinical Medicine and Surgery, Faculty of Veterinary Science, University of Agriculture, Faisalabad 38000, Pakistan)

  • Usman Tahir

    (Livestock and Dairy Development, Government of Punjab, Lahore 54100, Pakistan)

  • Iahtasham Khan

    (Section of Epidemiology and Public Health, University of Veterinary and Animal Sciences, Lahore, sub-campus Jhang, 12-Km Chiniot Road, Jhang 35200, Pakistan)

  • Muhammad Haleem Tayyab

    (Department of Clinical Medicine and Surgery, Faculty of Veterinary Science, University of Agriculture, Faisalabad 38000, Pakistan)

  • Sami Ullah

    (Department of Clinical Medicine and Surgery, Faculty of Veterinary Science, University of Agriculture, Faisalabad 38000, Pakistan)

  • Mashkoor Mohsin

    (Institute of Microbiology, University of Agriculture, Faisalabad 38000, Pakistan)

  • Muhammad Khalid Mansoor

    (Department of Microbiology, Faculty of Veterinary Science, Cholistan University of Veterinary and Animal Sciences, Bahawalpur 63100, Pakistan)

  • Stefan Schwarz

    (Institute of Microbiology and Epizootics, Freie Universität Berlin, 14163 Berlin, Germany)

  • Heinrich Neubauer

    (Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut, 07743 Jena, Germany)

Abstract

Bovine brucellosis remains a persistent infection in ruminants in Pakistan. A total of 828 (409 buffaloes and 419 cattle) sera were collected from 11 institutional-owned livestock farms in Punjab, Pakistan. The samples were tested by rose bengal plate agglutination test (RBPT) and indirect enzyme-linked immunosorbent assay (iELISA). The seroprevalence along with 95% confidence interval (CI) was determined. Univariable and multivariable analysis of the epidemiological background data was conducted and odds ratio (OR) was calculated to understand any association between the risk factors and the seroprevalence. An overall seroprevalence of 3.9% (Positive/Tested = 32/828) and 3.3% (27/828) was detected by RBPT and iELISA, respectively. The seroprevalence of 5.6% (CI 3.6–8.3) and 4.7%, (CI 2.8–7.2) and the odds ratio of 2.63 (CI 1.20–5.77) and 2.50 (CI 1.08–5.78) for testing positive by RBPT and iELISA, respectively were significantly higher ( p < 0.05) in buffaloes than in cattle. Breed, sex, history of abortion and retention of fetal membranes (RFM) in the animals were not found statistically significantly associated with the infection. RBPT and iELISA based results agreed almost perfect ( k = 0.877). In total, Brucella abortus -DNA (9/27) was amplified from seropositive samples by real-time polymerase chain reaction. This study identified for the first time the etiological agents of brucellosis at a molecular level at institutional-owned livestock farms in Pakistan.

Suggested Citation

  • Tariq Jamil & Falk Melzer & Muhammad Saqib & Asim Shahzad & Khushal Khan Kasi & Muhammad Hammad Hussain & Imaad Rashid & Usman Tahir & Iahtasham Khan & Muhammad Haleem Tayyab & Sami Ullah & Mashkoor M, 2020. "Serological and Molecular Detection of Bovine Brucellosis at Institutional Livestock Farms in Punjab, Pakistan," IJERPH, MDPI, vol. 17(4), pages 1-13, February.
    • Handle: RePEc:gam:jijerp:v:17:y:2020:i:4:p:1412-:d:323631
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