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Levels of Gene Expression of Immunological Biomarkers in Peri-Implant and Periodontal Tissues

Author

Listed:
  • Luciene Cristina Figueiredo

    (Dental Research Division, Guarulhos University, São Paulo 07023-070, Brazil)

  • Bruno Bueno-Silva

    (Dental Research Division, Guarulhos University, São Paulo 07023-070, Brazil)

  • Cristiana Fernandes Plutarco Nogueira

    (Dental Research Division, Guarulhos University, São Paulo 07023-070, Brazil)

  • Leonardo Carneiro Valadares

    (Dental Research Division, Guarulhos University, São Paulo 07023-070, Brazil)

  • Katia Marina Morilla Garcia

    (Dental Research Division, Guarulhos University, São Paulo 07023-070, Brazil)

  • Givelton Coimbra da Luz Filho

    (Dental Research Division, Guarulhos University, São Paulo 07023-070, Brazil)

  • Luciano Milanello

    (Dental Research Division, Guarulhos University, São Paulo 07023-070, Brazil)

  • Felipe Machado Esteves

    (Dental Research Division, Guarulhos University, São Paulo 07023-070, Brazil)

  • Jamil Awad Shibli

    (Dental Research Division, Guarulhos University, São Paulo 07023-070, Brazil)

  • Tamires Szeremeske Miranda

    (Dental Research Division, Guarulhos University, São Paulo 07023-070, Brazil)

Abstract

This study compared the gene expression of the immunoinflammatory markers interleukin (IL)-6, IL-1ß, and tumor necrosis factor alpha (TNF-α), the matrix metalloproteinases (MMP)-1, -2, -8, and -9, and the tissue inhibitors of matrix metalloproteases (TIMP)-1 and -2 in the gingival tissue of individuals with periodontal and peri-implant disease. The study population included individuals with four periodontal statuses: periodontal health (PH group, n = 20); periodontitis (P group, n = 20); peri-implant health (PIH group, n = 20), and peri-implantitis (PI group, n = 20). Gingival biopsies were collected from one tooth per patient according to the inclusion criteria of each group. The mRNA levels of IL-6, IL-1ß, TNF-α, MMP-1, MMP-2, MMP-8, MMP-9, TIMP-1, and TIMP-2 were evaluated by qPCR. The levels of IL-1ß were significantly higher in the PI group when compared to the other groups ( p < 0.05), while the levels of IL-6 were significantly higher in the groups with periodontal and peri-implant disease when compared with the healthy groups ( p < 0.05); however, the levels of IL-6 did not differ between the PI and P groups ( p > 0.05). For all other studied biomarkers, no significant differences were observed between groups ( p > 0.05). IL-6 and IL-1ß presented higher levels of mRNA in diseased periodontal and peri-implant tissues. However, the expression of metalloproteinases and their inhibitors did not differ between the different periodontal statuses.

Suggested Citation

  • Luciene Cristina Figueiredo & Bruno Bueno-Silva & Cristiana Fernandes Plutarco Nogueira & Leonardo Carneiro Valadares & Katia Marina Morilla Garcia & Givelton Coimbra da Luz Filho & Luciano Milanello , 2020. "Levels of Gene Expression of Immunological Biomarkers in Peri-Implant and Periodontal Tissues," IJERPH, MDPI, vol. 17(23), pages 1-9, December.
    • Handle: RePEc:gam:jijerp:v:17:y:2020:i:23:p:9100-:d:457584
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    References listed on IDEAS

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    1. Vittorio Checchi & Tatjana Maravic & Pierantonio Bellini & Luigi Generali & Ugo Consolo & Lorenzo Breschi & Annalisa Mazzoni, 2020. "The Role of Matrix Metalloproteinases in Periodontal Disease," IJERPH, MDPI, vol. 17(14), pages 1-13, July.
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