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Sensitivity of Planktonic Cells of Staphylococcus aureus to Elevated Hydrostatic Pressure as Affected by Mild Heat, Carvacrol, Nisin, and Caprylic Acid

Author

Listed:
  • Jyothi George

    (Public Health Microbiology Laboratory, Tennessee State University, Nashville, TN 37209, USA
    Department of Biological Sciences, Tennessee State University, Nashville, TN 37209, USA)

  • Sadiye Aras

    (Public Health Microbiology Laboratory, Tennessee State University, Nashville, TN 37209, USA
    Department of Biological Sciences, Tennessee State University, Nashville, TN 37209, USA)

  • Md Niamul Kabir

    (Public Health Microbiology Laboratory, Tennessee State University, Nashville, TN 37209, USA)

  • Sabrina Wadood

    (Public Health Microbiology Laboratory, Tennessee State University, Nashville, TN 37209, USA)

  • Shahid Chowdhury

    (Public Health Microbiology Laboratory, Tennessee State University, Nashville, TN 37209, USA)

  • Aliyar Cyrus Fouladkhah

    (Public Health Microbiology Laboratory, Tennessee State University, Nashville, TN 37209, USA
    Cooperative Extension Program, Tennessee State University, Nashville, TN 37209, USA)

Abstract

Current study investigated effects of elevated hydrostatic pressure exposure in the presence of mild heat and natural antimicrobials against Staphylococcus aureus . Hydrostatic pressure of 350 to 550 MPa with nisin (5000 IU/mL), carvacrol, or caprylic acid (0.5% v / v ) were applied for the reduction in four-strain mixture of S. aureus in HEPES buffer at 4 and 40 °C for up to 7 min. Results were statistically analyzed by ANOVA and D-values were additionally calculated using best-fitted linear model. Prior to exposure to treatments at 4 °C, counts of the pathogen were 7.95 ± 0.4 log CFU/mL and were reduced ( p < 0.05) to 6.44 ± 0.3 log CFU/mL after 7 min of treatment at 450 MPa. D-value associated with this treatment was 5.34 min (R 2 = 0.72). At 40 °C, counts were 8.21 ± 0.7 and 5.77 ± 0.3 log CFU/mL before and after the 7-min treatments, respectively. D-value associated with 40 °C treatment was 3.30 min (R 2 = 0.62). Application of the antimicrobials provided additional pathogen reduction augmentation for treatments < 5 min. The results of the current study could be incorporated for meeting regulatory requirements such as Food Code, HACCP, and Preventive Control for Human Food of Food Safety Modernization Act for assuring microbiological safety of products against this prevalent pathogen of public health concern.

Suggested Citation

  • Jyothi George & Sadiye Aras & Md Niamul Kabir & Sabrina Wadood & Shahid Chowdhury & Aliyar Cyrus Fouladkhah, 2020. "Sensitivity of Planktonic Cells of Staphylococcus aureus to Elevated Hydrostatic Pressure as Affected by Mild Heat, Carvacrol, Nisin, and Caprylic Acid," IJERPH, MDPI, vol. 17(19), pages 1-14, September.
    • Handle: RePEc:gam:jijerp:v:17:y:2020:i:19:p:7033-:d:419905
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    References listed on IDEAS

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    1. Sadiye Aras & Md Niamul Kabir & Shahid Chowdhury & Aliyar Cyrus Fouladkhah, 2020. "Augmenting the Pressure-Based Pasteurization of Listeria monocytogenes by Synergism with Nisin and Mild Heat," IJERPH, MDPI, vol. 17(2), pages 1-13, January.
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