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Pseudomonas fluorescens : A Bioaugmentation Strategy for Oil-Contaminated and Nutrient-Poor Soil

Author

Listed:
  • Eduardo Jahir Gutiérrez

    (Facultad de Ciencias Químico-Biológicas, Universidad Autónoma de Campeche, Av. Agustín Melgar S/N, Buena Vista, Campeche 24039, Mexico)

  • María del Rosario Abraham

    (División de Ciencias de la Vida, Departamento de Ingeniería en Alimentos, Universidad de Guanajuato, Hacienda El Copal Km 9, Carretera Irapuato-Silao; AP 311, Gto. Irapuato 36500, Mexico)

  • Juan Carlos Baltazar

    (División de Ingenierías, Departamento de Ingeniería en Minas, Universidad de Guanajuato, Metalurgia y Geología, Ex Had. De San Matias S/N. Col. San Javier, Gto. Guanajuato 36020, Mexico)

  • Guadalupe Vázquez

    (División de Ingenierías, Departmento de Ingeniería Civil y Ambiental, Universidad de Guanajuato, Av. Juárez N 77, Col. Centro, Guanajuato Gto. 36000, Mexico)

  • Eladio Delgadillo

    (División de Ingenierías, Departmento de Ingeniería Civil y Ambiental, Universidad de Guanajuato, Av. Juárez N 77, Col. Centro, Guanajuato Gto. 36000, Mexico)

  • David Tirado

    (División de Ingenierías, Departmento de Ingeniería Civil y Ambiental, Universidad de Guanajuato, Av. Juárez N 77, Col. Centro, Guanajuato Gto. 36000, Mexico)

Abstract

Bioremediation technology is one of the most profitable and sustainable strategies for remediating soils contaminated with hydrocarbons. This study focuses on assessing the influence of biostimulation and bioaugmentation with Pseudomonas fluorescens to contribute to the removal of total petroleum hydrocarbons (TPHs) of a soil. Laboratory studies were carried out (measurements of emitted CO 2 , surface tension, and residual TPH) to select the best bioaugmentation and biostimulation treatment. The sources of C, N, and P were glucose–yeast extract, NH 4 Cl–NaNO 3 , and K 2 HPO 4 –K 3 PO 4 , respectively. The effect of culture conditions on the reduction of TPH and respiratory activity was evaluated through a factorial design, 2 3 , in a solid culture system. After 80 days of incubation, it was observed that treatments of yeast extract–NH 4 Cl–K 2 HPO 4 (Y4) and glucose–NaNO 3 –K 3 PO 4 (Y5) presented a higher level of TPH removal (20.91% and 20.00% degradation of TPH, respectively). Biostimulation favors the production of biosurfactants, indirectly measured by the change in surface tension in the soil extracts. The treatments Y4 and Y5 showed a lower change value of the surface tension (23.15 and 23.30 mN·m −1 at 25 °C). A positive correlation was determined between the change in surface tension and the removal of TPH; hence there was a contribution of the biosurfactants produced to the removal of hydrocarbons.

Suggested Citation

  • Eduardo Jahir Gutiérrez & María del Rosario Abraham & Juan Carlos Baltazar & Guadalupe Vázquez & Eladio Delgadillo & David Tirado, 2020. "Pseudomonas fluorescens : A Bioaugmentation Strategy for Oil-Contaminated and Nutrient-Poor Soil," IJERPH, MDPI, vol. 17(19), pages 1-14, September.
    • Handle: RePEc:gam:jijerp:v:17:y:2020:i:19:p:6959-:d:417932
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    Citations

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    Cited by:

    1. Barbara Bertović & Monika Šabić Runjavec & Nolla Todorović & Ivan Zgrebec & Marija Vuković Domanovac, 2024. "Biotechnological Potential of Oil-Tolerant Strains for Possible Use in Bioremediation," Sustainability, MDPI, vol. 16(2), pages 1-16, January.
    2. Nurzat Totubaeva & Zhiide Tokpaeva & Janarbek Izakov & Mirlan Moldobaev, 2023. "Bioremediation approaches for oil contaminated soils in extremely high-mountainous conditions," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 69(4), pages 188-193.

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