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Effect of Urban Wastewater Discharge on the Abundance of Antibiotic Resistance Genes and Antibiotic-Resistant Escherichia coli in Two Italian Rivers

Author

Listed:
  • Fabrizio Pantanella

    (Department of Public Health and Infectious Diseases, University of Rome “La Sapienza”, Piazza A. Moro 5, 00185 Rome, Italy)

  • Itziar Lekunberri

    (Catalan Institute for Water Research (ICRA), Scientific and Technological Park of the University of Girona, 17003 Girona, Spain)

  • Antonella Gagliardi

    (Department of Public Health and Infectious Diseases, University of Rome “La Sapienza”, Piazza A. Moro 5, 00185 Rome, Italy)

  • Giuseppe Venuto

    (Department of Public Health and Infectious Diseases, University of Rome “La Sapienza”, Piazza A. Moro 5, 00185 Rome, Italy)

  • Alexandre Sànchez-Melsió

    (Catalan Institute for Water Research (ICRA), Scientific and Technological Park of the University of Girona, 17003 Girona, Spain)

  • Massimo Fabiani

    (Department of Public Health and Infectious Diseases, University of Rome “La Sapienza”, Piazza A. Moro 5, 00185 Rome, Italy)

  • José Luis Balcázar

    (Catalan Institute for Water Research (ICRA), Scientific and Technological Park of the University of Girona, 17003 Girona, Spain)

  • Serena Schippa

    (Department of Public Health and Infectious Diseases, University of Rome “La Sapienza”, Piazza A. Moro 5, 00185 Rome, Italy)

  • Maria De Giusti

    (Department of Public Health and Infectious Diseases, University of Rome “La Sapienza”, Piazza A. Moro 5, 00185 Rome, Italy)

  • Carles Borrego

    (Catalan Institute for Water Research (ICRA), Scientific and Technological Park of the University of Girona, 17003 Girona, Spain
    Group of Molecular Microbial Ecology, Institute of Aquatic Ecology, University of Girona, 17001 Girona, Spain
    These authors were co-principal investigators.)

  • Angelo Solimini

    (Department of Public Health and Infectious Diseases, University of Rome “La Sapienza”, Piazza A. Moro 5, 00185 Rome, Italy
    These authors were co-principal investigators.)

Abstract

Background: Wastewater treatment plants (WWTPs) are microbial factories aimed to reduce the amount of nutrients and pathogenic microorganisms in the treated wastewater before its discharge into the environment. We studied the impact of urban WWTP effluents on the abundance of antibiotic resistance genes (ARGs) and antibiotic-resistant Escherichia coli (AR- E. coli ) in the last stretch of two rivers (Arrone and Tiber) in Central Italy that differ in size and flow volume. Methods: Water samples were collected in three seasons upstream and downstream of the WWTP, at the WWTP outlet, and at sea sites near the river mouth, and analyzed for the abundance of ARGs by qPCR and AR- E. coli using cultivation followed by disk diffusion assays. Results: For all studied genes ( 16S rRNA , intI1 , sul1 , ermB , blaTEM , tetW and qnrS ), absolute concentrations were significantly higher in the Tiber than in the Arrone at all sampling sites, despite their collection date, but the prevalence of target ARGs within bacterial communities in both rivers was similar. The absolute concentrations of most ARGs were also generally higher in the WWTP effluent with median levels between log 4 and log 6 copies per ml but did not show differences along the studied stretches of rivers. Statistically significant site effect was found for E. coli phenotypic resistance to tetracycline and ciprofloxacin in the Arrone but not in the Tiber. Conclusions: In both rivers, diffuse or point pollution sources other than the studied WWTP effluents may account for the observed resistance pattern, although the Arrone appears as more sensitive to the wastewater impact considering its lower flow volume.

Suggested Citation

  • Fabrizio Pantanella & Itziar Lekunberri & Antonella Gagliardi & Giuseppe Venuto & Alexandre Sànchez-Melsió & Massimo Fabiani & José Luis Balcázar & Serena Schippa & Maria De Giusti & Carles Borrego & , 2020. "Effect of Urban Wastewater Discharge on the Abundance of Antibiotic Resistance Genes and Antibiotic-Resistant Escherichia coli in Two Italian Rivers," IJERPH, MDPI, vol. 17(18), pages 1-13, September.
    • Handle: RePEc:gam:jijerp:v:17:y:2020:i:18:p:6813-:d:415637
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    References listed on IDEAS

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    1. Stefania Marcheggiani & Emilo D'Ugo & Camilla Puccinelli & Roberto Giuseppetti & Anna Maria D'Angelo & Claudio Orlando Gualerzi & Roberto Spurio & Linda K. Medlin & Delphine Guillebault & Wilfried Wei, 2015. "Detection of Emerging and Re-Emerging Pathogens in Surface Waters Close to an Urban Area," IJERPH, MDPI, vol. 12(5), pages 1-23, May.
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