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Exercise- and Cold-Induced Human PGC-1α mRNA Isoform Specific Responses

Author

Listed:
  • Camille Larson

    (School of Health and Kinesiology, University of Nebraska at Omaha, Omaha, NE 68182, USA)

  • Megan Opichka

    (School of Health and Kinesiology, University of Nebraska at Omaha, Omaha, NE 68182, USA)

  • Mark L. McGlynn

    (School of Health and Kinesiology, University of Nebraska at Omaha, Omaha, NE 68182, USA)

  • Christopher W. Collins

    (School of Health and Kinesiology, University of Nebraska at Omaha, Omaha, NE 68182, USA)

  • Dustin Slivka

    (School of Health and Kinesiology, University of Nebraska at Omaha, Omaha, NE 68182, USA)

Abstract

Cold exposure in conjunction with aerobic exercise stimulates gene expression of PGC-1α , the master regulator of mitochondrial biogenesis. PGC-1α can be expressed as multiple isoforms due to alternative splicing mechanisms. Among these isoforms is NT-PGC-1α , which produces a truncated form of the PGC-1α protein, as well as isoforms derived from the first exon of the transcript, PGC-1α-a , PGC-1α-b , and PGC-1α-c . Relatively little is known about the individual responses of these isoforms to exercise and environmental temperature. Therefore, we determined the expression of PGC-1α isoforms following an acute bout of cycling in cold (C) and room temperature (RT) conditions. Nine male participants cycled for 1h at 65% Wmax at −2 °C and 20 °C. A muscle biopsy was taken from the vastus lateralis before and 3h post-exercise. RT-qPCR was used to analyze gene expression of PGC-1α isoforms. Gene expression of all PGC-1α isoforms increased due to the exercise intervention ( p < 0.05). Exercise and cold exposure induced a greater increase in gene expression for total PGC-1α ( p = 0.028) and its truncated isoform, NT-PGC-1α ( p = 0.034), but there was no temperature-dependent response in the other PGC-1α isoforms measured. It appears that NT-PGC-1α may have a significant contribution to the reported alterations in the exercise- and temperature-induced PGC-1α response.

Suggested Citation

  • Camille Larson & Megan Opichka & Mark L. McGlynn & Christopher W. Collins & Dustin Slivka, 2020. "Exercise- and Cold-Induced Human PGC-1α mRNA Isoform Specific Responses," IJERPH, MDPI, vol. 17(16), pages 1-10, August.
    • Handle: RePEc:gam:jijerp:v:17:y:2020:i:16:p:5740-:d:396328
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