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Probiotic Properties of Bacillus Strains Isolated from Stingless Bee ( Heterotrigona itama ) Honey Collected across Malaysia

Author

Listed:
  • Fatin Aina Zulkhairi Amin

    (Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia)

  • Suriana Sabri

    (Enzyme and Microbial Technology Research Center, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia
    Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia)

  • Maznah Ismail

    (Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia)

  • Kim Wei Chan

    (Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia)

  • Norsharina Ismail

    (Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia)

  • Norhaizan Mohd Esa

    (Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia
    Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia)

  • Mohd Azmi Mohd Lila

    (Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang 43400 UPM, Selangor, Malaysia)

  • Norhasnida Zawawi

    (Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia
    Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia)

Abstract

This study aimed to isolate, identify, and evaluate the probiotic properties of Bacillus species from honey of the stingless bee Heterotrigona itama . Bacillus spp. were isolated from five different H. itama meliponicultures, and the isolates were characterized through Gram-staining and a catalase test. Tolerance to acidic conditions and bile salt (0.3%), hydrophobicity, and autoaggregation tests were performed to assess the probiotic properties of the selected isolates, B. amyloliquefaciens HTI-19 and B. subtilis HTI-23. Both Bacillus isolates exhibited excellent antimicrobial activity against both Gram-positive and Gram-negative bacteria and possessed significantly high survival rates in 0.3% bile solution for 3 h. Their survival rates in acidic conditions were also comparable to a commercial probiotic strain, Lactobacillus rhamnosus GG. Interestingly, the hydrophobicity and autoaggregation percentage showed no significant difference from L. rhamnosus GG, a commercial probiotic strain. The results from this study suggest that B. amyloliquefaciens HTI-19 and B. subtilis HTI-23 isolated from stingless bee honey have considerably good probiotic properties. Therefore, more studies should be done to investigate the effects of these bacteria cultures on gastrointestinal health.

Suggested Citation

  • Fatin Aina Zulkhairi Amin & Suriana Sabri & Maznah Ismail & Kim Wei Chan & Norsharina Ismail & Norhaizan Mohd Esa & Mohd Azmi Mohd Lila & Norhasnida Zawawi, 2019. "Probiotic Properties of Bacillus Strains Isolated from Stingless Bee ( Heterotrigona itama ) Honey Collected across Malaysia," IJERPH, MDPI, vol. 17(1), pages 1-15, December.
    • Handle: RePEc:gam:jijerp:v:17:y:2019:i:1:p:278-:d:303532
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    References listed on IDEAS

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    1. Magdalena Pajor & Randy W. Worobo & Sławomir Milewski & Piotr Szweda, 2018. "The Antimicrobial Potential of Bacteria Isolated from Honey Samples Produced in the Apiaries Located in Pomeranian Voivodeship in Northern Poland," IJERPH, MDPI, vol. 15(9), pages 1-14, September.
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