IDEAS home Printed from https://ideas.repec.org/a/gam/jijerp/v16y2019i4p646-d208118.html
   My bibliography  Save this article

Isolation and Characterization of a Bacterial Strain Capable of Efficient Berberine Degradation

Author

Listed:
  • Shiyue Liu

    (School of Water Resources and Environment, China University of Geosciences (Beijing), 20 Chengfu Road, Haidian District, Beijing 100083, China
    Chinese Research Academy of Environmental Sciences, 8 Dayangfang, Chaoyang District, Beijing 100012, China)

  • Yi Zhang

    (Fudan University, 2005 Songhu Road, Yangpu District, Shanghai 200438, China)

  • Ping Zeng

    (Chinese Research Academy of Environmental Sciences, 8 Dayangfang, Chaoyang District, Beijing 100012, China)

  • Heli Wang

    (School of Water Resources and Environment, China University of Geosciences (Beijing), 20 Chengfu Road, Haidian District, Beijing 100083, China)

  • Yonghui Song

    (Chinese Research Academy of Environmental Sciences, 8 Dayangfang, Chaoyang District, Beijing 100012, China)

  • Juan Li

    (Chinese Research Academy of Environmental Sciences, 8 Dayangfang, Chaoyang District, Beijing 100012, China)

Abstract

Background: Berberine (BBR) is a pharmaceutical chemical with a broad antibacterial spectrum, and its biological treatment has been of research and practical interest. In this study, a pure bacterial strain B16 was isolated from the activated sludge in a pharmaceutical wastewater treatment plant. The aim of the study is to characterize the properties of the strain B16, especially its BBR degradation capability. Methods: The identification of strain B16 was conducted by visual observation, as well as biochemical and phylogenetic analysis. The degradation kinetics of strain B16 was tentatively described by Haldane model. Results: The strain B16 was 100% determined as a Sphingopyxis sp. The kinetic parameters of BBR degradation by strain B16 were as follows: Vmax 54.73 ± 5.54 mg (g MLSS · h) −1 , Km 66.68 ± 8.95 mg L −1 , and Ki 43.16 ± 5.92 mg L −1 , with an R 2 of 0.996. Stain B16 exhibited considerable capability of BBR degradation. BBR of initial concentration 40 mg L −1 could be completely degraded in 48 h under optimal conditions. Conclusions: strain B16 was the first pure culture found with the ability to totally mineralize BBR, indicating the potential of B16 application in real industrial processes.

Suggested Citation

  • Shiyue Liu & Yi Zhang & Ping Zeng & Heli Wang & Yonghui Song & Juan Li, 2019. "Isolation and Characterization of a Bacterial Strain Capable of Efficient Berberine Degradation," IJERPH, MDPI, vol. 16(4), pages 1-12, February.
    • Handle: RePEc:gam:jijerp:v:16:y:2019:i:4:p:646-:d:208118
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1660-4601/16/4/646/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1660-4601/16/4/646/
    Download Restriction: no
    ---><---

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jijerp:v:16:y:2019:i:4:p:646-:d:208118. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.