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Indoor Microbiome and Antibiotic Resistance on Floor Surfaces: An Exploratory Study in Three Different Building Types

Author

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  • Mridula Gupta

    (College of public Health, Division of Environmental Health Sciences, The Ohio State University, Columbus, OH 43210, USA
    These authors contributed equally to the work.)

  • Seungjun Lee

    (College of public Health, Division of Environmental Health Sciences, The Ohio State University, Columbus, OH 43210, USA
    These authors contributed equally to the work.)

  • Michael Bisesi

    (College of public Health, Division of Environmental Health Sciences, The Ohio State University, Columbus, OH 43210, USA)

  • Jiyoung Lee

    (College of public Health, Division of Environmental Health Sciences, The Ohio State University, Columbus, OH 43210, USA
    Department of Food Science and Technology, The Ohio State University, Columbus, OH 43210, USA)

Abstract

Floor materials in indoor environments are known to be reservoirs of microbes. We focused on examining bacterial community composition, antibiotic resistance (AR) and microbial source tracking (MST) of fecal bacteria on the floor surfaces. Swab samples were collected from carpet and vinyl floors in three different buildings (medical, veterinary, and office buildings) from high and low traffic areas. Bacterial communities were determined with 16S rRNA sequencing, and AR (tetracycline ( tet Q), sulfonamide, and carbapenem (KPC)) and MST (human-, canine-, avian-, and ruminant-specific fecal bacteria) were examined with quantitative polymerase chain reaction (PCR). The results show that Proteobacteria and Actinobacteria were the most abundant phyla. Traffic level significantly affected the number of operational taxonomic units. Traffic level was a key factor for distinctive bacterial community in the medical center. Targeted ARGs were detected from all buildings and tet Q concentration was related with traffic level, and KPC was only detected from the medical center. Most of the floor surfaces showed the presence of dog-specific fecal bacteria (83%) followed by bird-specific fecal bacteria (75%). The results suggest that traffic levels affected the bacterial levels and fecal contamination is prevalent on the floor surfaces. This is the first study that reports KPC presence on the floor surfaces.

Suggested Citation

  • Mridula Gupta & Seungjun Lee & Michael Bisesi & Jiyoung Lee, 2019. "Indoor Microbiome and Antibiotic Resistance on Floor Surfaces: An Exploratory Study in Three Different Building Types," IJERPH, MDPI, vol. 16(21), pages 1-11, October.
    • Handle: RePEc:gam:jijerp:v:16:y:2019:i:21:p:4160-:d:281151
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    References listed on IDEAS

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    1. Tairacan Augusto Pereira da Fonseca & Rodrigo Pessôa & Alvina Clara Felix & Sabri Saeed Sanabani, 2016. "Diversity of Bacterial Communities on Four Frequently Used Surfaces in a Large Brazilian Teaching Hospital," IJERPH, MDPI, vol. 13(2), pages 1-11, January.
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    Cited by:

    1. Nazima Habibi & Saif Uddin & Montaha Behbehani & Abu Salim Mustafa & Wadha Al-Fouzan & Hanan A. Al-Sarawi & Hussain Safar & Fatemah Alatar & Rima M. Z. Al Sawan, 2024. "Aerosol-Mediated Spread of Antibiotic Resistance Genes: Biomonitoring Indoor and Outdoor Environments," IJERPH, MDPI, vol. 21(8), pages 1-18, July.

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