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Use of MALDI-TOF MS to Discriminate between Biofilm-Producer and Non-Producer Strains of Staphylococcus epidermidis

Author

Listed:
  • Pina Caputo

    (Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology University of Campania “Luigi Vanvitelli”, 80138 Naples, Italy)

  • Maria Chiara Di Martino

    (Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology University of Campania “Luigi Vanvitelli”, 80138 Naples, Italy)

  • Brunella Perfetto

    (Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology University of Campania “Luigi Vanvitelli”, 80138 Naples, Italy)

  • Francesco Iovino

    (Division of General Surgery, Department of Cardiothoracic and Respiratory Science, University of Campania “Luigi Vanvitelli”, 80138 Naples, Italy)

  • Giovanna Donnarumma

    (Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology University of Campania “Luigi Vanvitelli”, 80138 Naples, Italy)

Abstract

For the management of Staphylococci coagulase-negative infection, often related to biofilm formation, rapid and accurate identification is necessary in choosing a correct antibiotic therapy. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) is becoming increasingly important for bacterial identification over traditional methods. Our aim was to validate the use of MALDI to discriminate Staphylococcus epidermidis biofilm-producing strains. Clinical strains coming from suture wires were identified and their protein profiles were compared to that obtained from two ATCC reference strains (biofilm producer and non-producer). MALDI identified the eighteen isolates as S. epidermidis , combining sixteen profiles with the biofilm producer and two with the non-producer, confirming the results of crystal violet assay. Our data highlight that MALDI can be considered a good tool to discriminate between biofilm-producer and non-producer strains of S. epidermidis , thus helping to establish an effective antibiotic therapy.

Suggested Citation

  • Pina Caputo & Maria Chiara Di Martino & Brunella Perfetto & Francesco Iovino & Giovanna Donnarumma, 2018. "Use of MALDI-TOF MS to Discriminate between Biofilm-Producer and Non-Producer Strains of Staphylococcus epidermidis," IJERPH, MDPI, vol. 15(8), pages 1-11, August.
    • Handle: RePEc:gam:jijerp:v:15:y:2018:i:8:p:1695-:d:162755
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