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Quorum Sensing versus Quenching Bacterial Isolates Obtained from MBR Plants Treating Leachates from Municipal Solid Waste

Author

Listed:
  • Albert Soler

    (Department of Genetics, Physiology and Microbiology, Complutense University of Madrid, 28040 Madrid, Spain)

  • Lucía Arregui

    (Department of Genetics, Physiology and Microbiology, Complutense University of Madrid, 28040 Madrid, Spain)

  • Miguel Arroyo

    (Department of Biochemistry and Molecular Biology I, Complutense University of Madrid, 28040 Madrid, Spain)

  • José Antonio Mendoza

    (Department of Chemical and Nuclear Engineering, Polytechnic University of Valencia, 46022 Valencia, Spain)

  • Andrea Muras

    (Department of Microbiology and Parasitology—CIBUS, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain)

  • Cristina Álvarez

    (URBASER S.A., Camino de Hormigueras, 171, 28031 Madrid, Spain)

  • Cristina García-Vera

    (URBASER S.A., Camino de Hormigueras, 171, 28031 Madrid, Spain)

  • Domingo Marquina

    (Department of Genetics, Physiology and Microbiology, Complutense University of Madrid, 28040 Madrid, Spain)

  • Antonio Santos

    (Department of Genetics, Physiology and Microbiology, Complutense University of Madrid, 28040 Madrid, Spain)

  • Susana Serrano

    (Department of Genetics, Physiology and Microbiology, Complutense University of Madrid, 28040 Madrid, Spain)

Abstract

Quorum sensing (QS) is a mechanism dependent on bacterial density. This coordinated process is mediated by the synthesis and the secretion of signal molecules, called autoinducers (AIs). N -acyl-homoserine lactones (AHLs) are the most common AIs that are used by Gram-negative bacteria and are involved in biofilm formation. Quorum Quenching (QQ) is the interference of QS by producing hydrolyzing enzymes, among other strategies. The main objective of the present study was to identify QS and QQ strains from MBR wastewater treatment plants. A total of 99 strains were isolated from two Spanish plants that were intended to treat leachate from municipal solid waste. Five AHL producers were detected using AHL biosensor strains ( Chromobacterium violaceum CV026 and Agrobacterium tumefaciens NT1). Fifteen strains of seventy-one Gram-positive were capable of eliminating or reducing at least one AHL activity. The analysis of 16S rRNA gene sequence showed the importance of the Pseudomonas genus in the production of biofilms and the relevance of the genus Bacillus in the disruption of the QS mechanism, in which the potential activity of lactonase or acylase enzymes was investigated with the aim to contribute to solve biofouling problems and to increase the useful lifespan of membranes.

Suggested Citation

  • Albert Soler & Lucía Arregui & Miguel Arroyo & José Antonio Mendoza & Andrea Muras & Cristina Álvarez & Cristina García-Vera & Domingo Marquina & Antonio Santos & Susana Serrano, 2018. "Quorum Sensing versus Quenching Bacterial Isolates Obtained from MBR Plants Treating Leachates from Municipal Solid Waste," IJERPH, MDPI, vol. 15(5), pages 1-16, May.
    • Handle: RePEc:gam:jijerp:v:15:y:2018:i:5:p:1019-:d:147711
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