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Comparison of Commensal Escherichia coli Isolates from Adults and Young Children in Lubuskie Province, Poland: Virulence Potential, Phylogeny and Antimicrobial Resistance

Author

Listed:
  • Ewa Bok

    (Department of Microbiology and Genetics, Faculty of Biological Sciences, University of Zielona Góra, 65-561 Zielona Góra, Poland)

  • Justyna Mazurek

    (Department of Microbiology and Genetics, Faculty of Biological Sciences, University of Zielona Góra, 65-561 Zielona Góra, Poland)

  • Andrzej Myc

    (Department of Microbiology and Genetics, Faculty of Biological Sciences, University of Zielona Góra, 65-561 Zielona Góra, Poland
    Laboratory of Virology, Department of Immunology of Infectious Diseases, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland
    Michigan Nanotechnology Institute for Medicine and Biological Sciences, University of Michigan Medical School, Ann Arbor, MI 48109-5648, USA)

  • Michał Stosik

    (Department of Microbiology and Genetics, Faculty of Biological Sciences, University of Zielona Góra, 65-561 Zielona Góra, Poland)

  • Magdalena Wojciech

    (Department of Mathematical Statistics and Econometrics, Faculty of Mathematics, Computer Science and Econometrics, University of Zielona Góra, 65-516 Zielona Góra, Poland)

  • Katarzyna Baldy-Chudzik

    (Department of Microbiology and Genetics, Faculty of Biological Sciences, University of Zielona Góra, 65-561 Zielona Góra, Poland)

Abstract

Commensal Escherichia coli population is a dynamic structure which may be important in the pathogenesis of extraintestinal infections. The aim of this study was the comparison of genetic diversity of commensal E. coli isolates from two age group—adults and young children. E. coli strains were isolated on MacConkey agar and identified by biochemical tests. Determination of four major phylogenetic groups, identification of virulence genes and antimicrobial resistance determinants were performed by using multiplex or simplex PCR. Phenotypic analysis of resistance was based on disc-diffusion method. The prevalence of virulence genes was significantly higher among isolates from adults than from young children. Phylogroup B2 predominated among E. coli from adults, whereas phylogroup A was the most common in isolates from young children. The analyses of antimicrobial resistance revealed that resistance to at least one antimicrobial agent and multidrug-resistance were detected significantly more frequent in the isolates from adults than from young children. This study documented that the commensal E. coli isolates from adults showed greater genetic diversity than from young children and constitutes a substantial reservoir of the virulence genes typical for extraintestinal pathogenic E. coli .

Suggested Citation

  • Ewa Bok & Justyna Mazurek & Andrzej Myc & Michał Stosik & Magdalena Wojciech & Katarzyna Baldy-Chudzik, 2018. "Comparison of Commensal Escherichia coli Isolates from Adults and Young Children in Lubuskie Province, Poland: Virulence Potential, Phylogeny and Antimicrobial Resistance," IJERPH, MDPI, vol. 15(4), pages 1-19, March.
    • Handle: RePEc:gam:jijerp:v:15:y:2018:i:4:p:617-:d:138463
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