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Diversity and Antimicrobial Resistance Genotypes in Non-Typhoidal Salmonella Isolates from Poultry Farms in Uganda

Author

Listed:
  • Terence Odoch

    (Department of Bio-security, Ecosystems and Veterinary Public Health, College of Veterinary Medicine, Animal Resources and Biosecurity (COVAB), Makerere University, P.O. Box 7062, Kampala, Uganda)

  • Camilla Sekse

    (Norwegian Veterinary Institute, 0106 Oslo, Norway)

  • Trine M. L’Abee-Lund

    (Department of Food Safety and Infection Biology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences (NMBU), 0454 Oslo, Norway)

  • Helge Christoffer Høgberg Hansen

    (Department of Food Safety and Infection Biology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences (NMBU), 0454 Oslo, Norway)

  • Clovice Kankya

    (Department of Bio-security, Ecosystems and Veterinary Public Health, College of Veterinary Medicine, Animal Resources and Biosecurity (COVAB), Makerere University, P.O. Box 7062, Kampala, Uganda)

  • Yngvild Wasteson

    (Department of Food Safety and Infection Biology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences (NMBU), 0454 Oslo, Norway)

Abstract

Non-typhoidal Salmonella (NTS) are foodborne pathogens of global public health significance. The aim of this study was to subtype a collection of 85 NTS originating from poultry farms in Uganda, and to evaluate a subgroup of phenotypically resistant isolates for common antimicrobial resistance genes and associated integrons. All isolates were subtyped by pulsed-field gel electrophoresis (PFGE). Phenotypically resistant isolates ( n = 54) were screened by PCR for the most relevant AMR genes corresponding to their phenotypic resistance pattern, and all 54 isolates were screened by PCR for the presence of integron class 1 and 2 encoding genes. These genes are known to commonly encode resistance to ampicillin, tetracycline, ciprofloxacin, trimethoprim, sulfonamide and chloramphenicol. PFGE revealed 15 pulsotypes representing 11 serotypes from 75 isolates, as 10 were non-typable. Thirty one (57.4%) of the 54 resistant isolates carried at least one of the seven genes ( bla TEM-1, cmlA, tetA, qnrS, sul1, dhfrI, dhfrVII) identified by PCR and six (11%) carried class 1 integrons. This study has shown that a diversity of NTS-clones are present in Ugandan poultry farm settings, while at the same time similar NTS-clones occur in different farms and areas. The presence of resistance genes to important antimicrobials used in human and veterinary medicine has been demonstrated, hence the need to strengthen strategies to combat antimicrobial resistance at all levels.

Suggested Citation

  • Terence Odoch & Camilla Sekse & Trine M. L’Abee-Lund & Helge Christoffer Høgberg Hansen & Clovice Kankya & Yngvild Wasteson, 2018. "Diversity and Antimicrobial Resistance Genotypes in Non-Typhoidal Salmonella Isolates from Poultry Farms in Uganda," IJERPH, MDPI, vol. 15(2), pages 1-14, February.
    • Handle: RePEc:gam:jijerp:v:15:y:2018:i:2:p:324-:d:131684
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    Citations

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    Cited by:

    1. Paula Rogovski & Raphael da Silva & Rafael Dorighello Cadamuro & Estêvão Brasiliense de Souza & Beatriz Pereira Savi & Aline Viancelli & William Michelon & Deisi Cristina Tápparo & Helen Treichel & Da, 2021. "Salmonella enterica Serovar Enteritidis Control in Poultry Litter Mediated by Lytic Bacteriophage Isolated from Swine Manure," IJERPH, MDPI, vol. 18(16), pages 1-9, August.
    2. Ashok J. Tamhankar & Cecilia Stålsby Lundborg, 2019. "Antimicrobials and Antimicrobial Resistance in the Environment and Its Remediation: A Global One Health Perspective," IJERPH, MDPI, vol. 16(23), pages 1-7, November.

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