IDEAS home Printed from https://ideas.repec.org/a/gam/jijerp/v13y2016i6p576-d71770.html
   My bibliography  Save this article

Use of a Sampling Area-Adjusted Adenosine Triphosphate Bioluminescence Assay Based on Digital Image Quantification to Assess the Cleanliness of Hospital Surfaces

Author

Listed:
  • Yu-Huai Ho

    (Division of Infection Diseases, Department of Internal Medicine, Buddhist Tzu Chi General Hospital and Tzu Chi University; Hualien 97004, Taiwan)

  • Lih-Shinn Wang

    (Division of Infection Diseases, Department of Internal Medicine, Buddhist Tzu Chi General Hospital and Tzu Chi University; Hualien 97004, Taiwan)

  • Hui-Li Jiang

    (Unit of Infection Control and Management, Buddhist Tzu Chi General Hospital, Hualien 97004, Taiwan)

  • Chih-Hui Chang

    (Department and Graduate Institute of Public Health, Tzu Chi University, Hualien 97004, Taiwan)

  • Chia-Jung Hsieh

    (Department and Graduate Institute of Public Health, Tzu Chi University, Hualien 97004, Taiwan)

  • Dan-Chi Chang

    (Department and Graduate Institute of Public Health, Tzu Chi University, Hualien 97004, Taiwan)

  • Hsin-Yu Tu

    (Department and Graduate Institute of Public Health, Tzu Chi University, Hualien 97004, Taiwan)

  • Tan-Yun Chiu

    (Department and Graduate Institute of Public Health, Tzu Chi University, Hualien 97004, Taiwan)

  • Huei-Jen Chao

    (Department of Laboratory Medicine, Buddhist Tzu Chi General Hospital, Hualien 97004, Taiwan)

  • Chun-Chieh Tseng

    (Department and Graduate Institute of Public Health, Tzu Chi University, Hualien 97004, Taiwan)

Abstract

Contaminated surfaces play an important role in the transmission of pathogens. We sought to establish a criterion that could indicate “cleanliness” using a sampling area–adjusted adenosine triphosphate (ATP) assay. In the first phase of the study, target surfaces were selected for swab sampling before and after daily cleaning; then, an aerobic colony count (ACC) plate assay of bacteria and antibiotic-resistant bacteria was conducted. ATP swabs were also tested, and the ATP readings were reported as relative light units (RLUs). The results of the ACC and ATP assays were adjusted according to the sampling area. During the second phase of the study, a new cleaning process employing sodium dichloroisocyanurate (NaDCC) was implemented for comparison. Using the criterion of 2.5 colony-forming units (CFU)/cm 2 , 45% of the sampled sites were successfully cleaned during phase one of the study. During phase two, the pass rates of the surface samples (64%) were significantly improved, except under stringent (5 RLU/cm 2 ) and lax (500 RLU) ATP criteria. Using receiver-operating characteristic curve analysis, the best cut-off point for an area-adjusted ATP level was 7.34 RLU/cm 2 , which corresponded to culture-assay levels of <2.5 CFU/cm 2 . An area adjustment of the ATP assay improved the degree of correlation with the ACC-assay results from weak to moderate.

Suggested Citation

  • Yu-Huai Ho & Lih-Shinn Wang & Hui-Li Jiang & Chih-Hui Chang & Chia-Jung Hsieh & Dan-Chi Chang & Hsin-Yu Tu & Tan-Yun Chiu & Huei-Jen Chao & Chun-Chieh Tseng, 2016. "Use of a Sampling Area-Adjusted Adenosine Triphosphate Bioluminescence Assay Based on Digital Image Quantification to Assess the Cleanliness of Hospital Surfaces," IJERPH, MDPI, vol. 13(6), pages 1-12, June.
    • Handle: RePEc:gam:jijerp:v:13:y:2016:i:6:p:576-:d:71770
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1660-4601/13/6/576/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1660-4601/13/6/576/
    Download Restriction: no
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Elizabeth C. Christenson & Ryan Cronk & Helen Atkinson & Aayush Bhatt & Emilio Berdiel & Michelle Cawley & Grace Cho & Collin Knox Coleman & Cailee Harrington & Kylie Heilferty & Don Fejfar & Emily J., 2021. "Evidence Map and Systematic Review of Disinfection Efficacy on Environmental Surfaces in Healthcare Facilities," IJERPH, MDPI, vol. 18(21), pages 1-22, October.
    2. Patryk Tarka & Aneta Nitsch-Osuch, 2020. "No-Touch Automated Disinfection System for Decontamination of Surfaces in Hospitals," IJERPH, MDPI, vol. 17(14), pages 1-10, July.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jijerp:v:13:y:2016:i:6:p:576-:d:71770. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.