IDEAS home Printed from https://ideas.repec.org/a/gam/jijerp/v13y2016i5p461-d69283.html
   My bibliography  Save this article

Use of Walnut Shell Powder to Inhibit Expression of Fe 2+ -Oxidizing Genes of Acidithiobacillus Ferrooxidans

Author

Listed:
  • Yuhui Li

    (Department of Biological and Environmental Engineering, Hefei University, Hefei 230601, China)

  • Yehao Liu

    (School of Public Health, Anhui Medical University, Hefei 230032, China)

  • Huifang Tan

    (Department of Biological and Environmental Engineering, Hefei University, Hefei 230601, China)

  • Yifeng Zhang

    (Department of Biological and Environmental Engineering, Hefei University, Hefei 230601, China)

  • Mei Yue

    (Department of Biological and Environmental Engineering, Hefei University, Hefei 230601, China)

Abstract

Acidithiobacillus ferrooxidans is a Gram-negative bacterium that obtains energy by oxidizing Fe 2+ or reduced sulfur compounds. This bacterium contributes to the formation of acid mine drainage (AMD). This study determined whether walnut shell powder inhibits the growth of A. ferrooxidans . First, the effects of walnut shell powder on Fe 2+ oxidization and H + production were evaluated. Second, the chemical constituents of walnut shell were isolated to determine the active ingredient(s). Third, the expression of Fe 2+ -oxidizing genes and rus operon genes was investigated using real-time polymerase chain reaction. Finally, growth curves were plotted, and a bioleaching experiment was performed to confirm the active ingredient(s) in walnut shells. The results indicated that both walnut shell powder and the phenolic fraction exert high inhibitory effects on Fe 2+ oxidation and H + production by A. ferrooxidans cultured in standard 9K medium. The phenolic components exert their inhibitory effects by down-regulating the expression of Fe 2+ -oxidizing genes and rus operon genes, which significantly decreased the growth of A. ferrooxidans . This study revealed walnut shell powder to be a promising substance for controlling AMD.

Suggested Citation

  • Yuhui Li & Yehao Liu & Huifang Tan & Yifeng Zhang & Mei Yue, 2016. "Use of Walnut Shell Powder to Inhibit Expression of Fe 2+ -Oxidizing Genes of Acidithiobacillus Ferrooxidans," IJERPH, MDPI, vol. 13(5), pages 1-12, April.
    • Handle: RePEc:gam:jijerp:v:13:y:2016:i:5:p:461-:d:69283
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1660-4601/13/5/461/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1660-4601/13/5/461/
    Download Restriction: no
    ---><---

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jijerp:v:13:y:2016:i:5:p:461-:d:69283. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.