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Supraphysiological Levels of Quercetin Glycosides are Required to Alter Mineralization in Saos2 Cells

Author

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  • Leslie A. Nash

    (Department of Health Sciences, Faculty of Applied Health Sciences, Brock University, 1812 Sir Isaac Brock Way, St. Catharines, ON L2S 3A1, Canada
    Centre for Bone and Muscle Health, Brock University, 1812 Sir Isaac Brock Way, St. Catharines, ON L2S 3A1, Canada)

  • Sandra J. Peters

    (Centre for Bone and Muscle Health, Brock University, 1812 Sir Isaac Brock Way, St. Catharines, ON L2S 3A1, Canada
    Department of Kinesiology, Faculty of Applied Health Sciences, Brock University, 1812 Sir Isaac Brock Way, St. Catharines, ON L2S 3A1, Canada
    These authors contributed equally to this work.)

  • Philip J. Sullivan

    (Department of Kinesiology, Faculty of Applied Health Sciences, Brock University, 1812 Sir Isaac Brock Way, St. Catharines, ON L2S 3A1, Canada
    These authors contributed equally to this work.)

  • Wendy E. Ward

    (Department of Health Sciences, Faculty of Applied Health Sciences, Brock University, 1812 Sir Isaac Brock Way, St. Catharines, ON L2S 3A1, Canada
    Centre for Bone and Muscle Health, Brock University, 1812 Sir Isaac Brock Way, St. Catharines, ON L2S 3A1, Canada
    Department of Kinesiology, Faculty of Applied Health Sciences, Brock University, 1812 Sir Isaac Brock Way, St. Catharines, ON L2S 3A1, Canada)

Abstract

Flavonoid intake is positively correlated to bone mineral density (BMD) in women. Flavonoids such as quercetin exhibit strong anti-oxidant and anti-inflammatory activity that may be beneficial for bone health. Quercetin, previously shown to positively influence osteoblasts, is metabolized into glycosides including rutin and hyperoside. We compared the effects of these glycosides on mineralization in human osteoblast (Saos2) cells. Administration of rutin (≥25 µM) and hyperoside (≥5 µM) resulted in higher mineral content, determined using the alizarin red assay. This was accompanied by higher alkaline phosphatase activity with no cell toxicity. The expression of osteopontin, sclerostin, TNFα and IL6, known stimuli for decreasing osteoblast activity, were reduced with the addition of rutin or hyperoside. In summary, rutin and hyperoside require supraphysiological levels, when administered individually, to positively influence osteoblast activity. This information may be useful in developing nutraceuticals to support bone health.

Suggested Citation

  • Leslie A. Nash & Sandra J. Peters & Philip J. Sullivan & Wendy E. Ward, 2016. "Supraphysiological Levels of Quercetin Glycosides are Required to Alter Mineralization in Saos2 Cells," IJERPH, MDPI, vol. 13(5), pages 1-11, April.
    • Handle: RePEc:gam:jijerp:v:13:y:2016:i:5:p:460-:d:69191
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