Author
Listed:
- Longjie Zhang
(Anhui Key Laboratory of Tobacco Chemistry, Technology Center, China Tobacco Anhui Industrial CO., LTD., Hefei 230088, China
State Key Laboratory of Tea Plant Biology and Utilization, School of Tea & Food Science, Anhui Agricultural University, Hefei 230036, China)
- Min Ning
(Anhui Key Laboratory of Tobacco Chemistry, Technology Center, China Tobacco Anhui Industrial CO., LTD., Hefei 230088, China)
- Yingbo Xu
(Anhui Key Laboratory of Tobacco Chemistry, Technology Center, China Tobacco Anhui Industrial CO., LTD., Hefei 230088, China)
- Chenghui Wang
(Anhui Key Laboratory of Tobacco Chemistry, Technology Center, China Tobacco Anhui Industrial CO., LTD., Hefei 230088, China)
- Guangshan Zhao
(State Key Laboratory of Tea Plant Biology and Utilization, School of Tea & Food Science, Anhui Agricultural University, Hefei 230036, China)
- Qingqing Cao
(State Key Laboratory of Tea Plant Biology and Utilization, School of Tea & Food Science, Anhui Agricultural University, Hefei 230036, China)
- Jinsong Zhang
(State Key Laboratory of Tea Plant Biology and Utilization, School of Tea & Food Science, Anhui Agricultural University, Hefei 230036, China)
Abstract
The present study investigated the influence of the cigarette smoke extract (CSE) on mammalian thioredoxin reductase (TrxR) activity. TrxR is a selenoenzyme with a selenocysteine (Sec) residue exposed on the enzyme’s surface. This unique Sec residue is particularly susceptible to modification by numerous types of electrophiles, leading to inactivation of TrxR and consequent cytotoxicity. Cigarette smoke contains various electrophiles, and the present study showed that CSE could inhibit intracellular TrxR through causing crosslinking and alkylation of TrxR1. TrxR inhibitory capacities of various CSEs were evaluated by using mouse-liver homogenate. Among the CSEs prepared from 18 commercial cigarette brands, TrxR inhibitory capacities of the maximum and the minimum had a 2.5-fold difference. Importantly, CSE’s inhibitory capacity greatly paralleled its cytotoxic potency in all cell lines used. Compared to cytotoxic assays, which have been widely used for evaluating cigarette toxicity but are not suitable for simultaneously examining a large number of cigarette samples, the present method was simple and rapid with a high-throughput feature and thus could be used as an auxiliary means to predict the cytotoxicity of a large number of cigarette samples, making it possible to extensively screen numerous agricultural and industrial measures that potentially affect cigarette safety.
Suggested Citation
Longjie Zhang & Min Ning & Yingbo Xu & Chenghui Wang & Guangshan Zhao & Qingqing Cao & Jinsong Zhang, 2016.
"Predicting the Cytotoxic Potency of Cigarette Smoke by Assessing the Thioredoxin Reductase Inhibitory Capacity of Cigarette Smoke Extract,"
IJERPH, MDPI, vol. 13(3), pages 1-13, March.
Handle:
RePEc:gam:jijerp:v:13:y:2016:i:3:p:348-:d:66186
Download full text from publisher
Corrections
All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jijerp:v:13:y:2016:i:3:p:348-:d:66186. See general information about how to correct material in RePEc.
If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.
We have no bibliographic references for this item. You can help adding them by using this form .
If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.
For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .
Please note that corrections may take a couple of weeks to filter through
the various RePEc services.