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Low-Dose Cadmium Upregulates VEGF Expression in Lung Adenocarcinoma Cells

Author

Listed:
  • Fuhong Liu

    (Medical Research Center, Shandong Provincial Qianfoshan Hospital, Shandong University, 16766 Jingshi Road, Jinan 250014, Shandong, China
    These authors contributed equally to the work.)

  • Bei Wang

    (Department of Geriatric Medicine, Shandong Provincial Qianfoshan Hospital, Shandong University, 16766 Jingshi Road, Jinan 250014, Shandong, China
    These authors contributed equally to the work.)

  • Liqun Li

    (Medical Research Center, Shandong Provincial Qianfoshan Hospital, Shandong University, 16766 Jingshi Road, Jinan 250014, Shandong, China)

  • Fengyun Dong

    (Medical Research Center, Shandong Provincial Qianfoshan Hospital, Shandong University, 16766 Jingshi Road, Jinan 250014, Shandong, China)

  • Xiaocui Chen

    (Medical Research Center, Shandong Provincial Qianfoshan Hospital, Shandong University, 16766 Jingshi Road, Jinan 250014, Shandong, China)

  • Yan Li

    (Children’s Health Center, Shandong Provincial Qianfoshan Hospital, Shandong University, 16766 Jingshi Road, Jinan 250014, Shandong, China)

  • Xiuzhen Dong

    (School of Biomedical Engineering, Fourth Military Medical University, Changle West Road No. 169, Xi’an 710032, Shanxi, China)

  • Youichiro Wada

    (The Research Center for Advanced Science and Technology, and Isotope Science Center, The University of Tokyo, 2-11-16, Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan)

  • Carolyn M. Kapron

    (Department of Biology, Trent University, Peterborough, Ontario K9J 7B8, Canada)

  • Ju Liu

    (Medical Research Center, Shandong Provincial Qianfoshan Hospital, Shandong University, 16766 Jingshi Road, Jinan 250014, Shandong, China)

Abstract

Cadmium (Cd) is a heavy metal and environmental toxin. Exposure to Cd has been associated with a variety of human cancers. In this study, we performed in vitro assays to examine the effects of cadmium chloride (CdCl 2 ) on A549 cells, a human lung adenocarcinoma cell line. Cd does not affect proliferation, migration, or apoptosis of A549 cells at concentrations of 0.1–10 μM. At 0.5 and 1 μM, Cd increases the expression of vascular endothelial growth factor (VEGF) ( p < 0.05, p < 0.01, respectively), but not basic fibroblast growth factor (b-FGF) in A549 cells. The conditioned media were collected from the A549 cells treated with 1 μM Cd and were co-cultured with human umbilical vein endothelial cells (HUVECs). Upon treatment with the conditioned media, the proliferation and migration of HUVECs significantly increased ( p < 0.01, p < 0.05, respectively), while apoptosis remained unchanged. In addition, 1 μM Cd increases the level of hypoxia inducible factor 1-α (HIF1-α), which is a positive regulator of VEGF expression. Although low-dose Cd does not directly affect the growth of lung adenocarcinoma cells, it might facilitate the development of tumors through its pro-angiogenic effects.

Suggested Citation

  • Fuhong Liu & Bei Wang & Liqun Li & Fengyun Dong & Xiaocui Chen & Yan Li & Xiuzhen Dong & Youichiro Wada & Carolyn M. Kapron & Ju Liu, 2015. "Low-Dose Cadmium Upregulates VEGF Expression in Lung Adenocarcinoma Cells," IJERPH, MDPI, vol. 12(9), pages 1-14, August.
    • Handle: RePEc:gam:jijerp:v:12:y:2015:i:9:p:10508-10521:d:54915
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    Cited by:

    1. Xiaocui Chen & Jing Li & Zuowang Cheng & Yinghua Xu & Xia Wang & Xiaorui Li & Dongmei Xu & Carolyn M. Kapron & Ju Liu, 2016. "Low Dose Cadmium Inhibits Proliferation of Human Renal Mesangial Cells via Activation of the JNK Pathway," IJERPH, MDPI, vol. 13(10), pages 1-12, October.

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