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Molecular Detection of a Potentially Toxic Diatom Species

Author

Listed:
  • Bidhan Chandra Dhar

    (Laboratory of Genetics, School of Biosciences and Veterinary Medicine, University of Camerino, Camerino 62032, Italy)

  • Lucia Cimarelli

    (Laboratory of Genetics, School of Biosciences and Veterinary Medicine, University of Camerino, Camerino 62032, Italy)

  • Kumar Saurabh Singh

    (Laboratory of Genetics, School of Biosciences and Veterinary Medicine, University of Camerino, Camerino 62032, Italy)

  • Letizia Brandi

    (Laboratory of Genetics, School of Biosciences and Veterinary Medicine, University of Camerino, Camerino 62032, Italy)

  • Anna Brandi

    (Laboratory of Genetics, School of Biosciences and Veterinary Medicine, University of Camerino, Camerino 62032, Italy)

  • Camilla Puccinelli

    (Environmental, Quality and Fishfarm Unit, Environment & Primary Prevention Department, Istituto Superiore di Sanità, Viale Regina Elena, Rome 299, Italy)

  • Stefania Marcheggiani

    (Environmental, Quality and Fishfarm Unit, Environment & Primary Prevention Department, Istituto Superiore di Sanità, Viale Regina Elena, Rome 299, Italy)

  • Roberto Spurio

    (Laboratory of Genetics, School of Biosciences and Veterinary Medicine, University of Camerino, Camerino 62032, Italy)

Abstract

A few diatom species produce toxins that affect human and animal health. Among these, members of the Pseudo-nitzschia genus were the first diatoms unambiguously identified as producer of domoic acid, a neurotoxin affecting molluscan shell-fish, birds, marine mammals, and humans. Evidence exists indicating the involvement of another diatom genus, Amphora, as a potential producer of domoic acid. We present a strategy for the detection of the diatom species Amphora coffeaeformis based on the development of species-specific oligonucleotide probes and their application in microarray hybridization experiments. This approach is based on the use of two marker genes highly conserved in all diatoms, but endowed with sufficient genetic divergence to discriminate diatoms at the species level. A region of approximately 450 bp of these previously unexplored marker genes, coding for elongation factor 1-a (eEF1-a) and silicic acid transporter ( SIT ), was used to design oligonucleotide probes that were tested for specificity in combination with the corresponding fluorescently labeled DNA targets. The results presented in this work suggest a possible use of this DNA chip technology for the selective detection of A. coffeaeformis in environmental settings where the presence of this potential toxin producer may represent a threat to human and animal health. In addition, the same basic approach can be adapted to a wider range of diatoms for the simultaneous detection of microorganisms used as biomarkers of different water quality levels.

Suggested Citation

  • Bidhan Chandra Dhar & Lucia Cimarelli & Kumar Saurabh Singh & Letizia Brandi & Anna Brandi & Camilla Puccinelli & Stefania Marcheggiani & Roberto Spurio, 2015. "Molecular Detection of a Potentially Toxic Diatom Species," IJERPH, MDPI, vol. 12(5), pages 1-21, May.
    • Handle: RePEc:gam:jijerp:v:12:y:2015:i:5:p:4921-4941:d:49219
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    References listed on IDEAS

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    1. Dörthe C. Müller-Navarra & Michael T. Brett & Anne M. Liston & Charles R. Goldman, 2000. "A highly unsaturated fatty acid predicts carbon transfer between primary producers and consumers," Nature, Nature, vol. 403(6765), pages 74-77, January.
    2. Chris Bowler & Andrew E. Allen & Jonathan H. Badger & Jane Grimwood & Kamel Jabbari & Alan Kuo & Uma Maheswari & Cindy Martens & Florian Maumus & Robert P. Otillar & Edda Rayko & Asaf Salamov & Klaas , 2008. "The Phaeodactylum genome reveals the evolutionary history of diatom genomes," Nature, Nature, vol. 456(7219), pages 239-244, November.
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    Cited by:

    1. Stefania Marcheggiani & Roberto Spurio & Lucia Cimarelli & Duarte Tito & Laura Mancini, 2015. "Scientific Symposium “Small Solution for Big Water-Related Problems: Innovative Microarrays and Small Sensors to Cope with Water Quality and Food Security”," IJERPH, MDPI, vol. 12(12), pages 1-9, December.
    2. Lucia Cimarelli & Kumar Saurabh Singh & Nguyen Thi Nhu Mai & Bidhan Chandra Dhar & Anna Brandi & Letizia Brandi & Roberto Spurio, 2015. "Molecular Tools for the Selective Detection of Nine Diatom Species Biomarkers of Various Water Quality Levels," IJERPH, MDPI, vol. 12(5), pages 1-20, May.

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