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Prevalence and Molecular Characteristics of Waterborne Pathogen Legionella in Industrial Cooling Tower Environments

Author

Listed:
  • Lijie Li

    (Shijiazhuang Center for Disease Control and Prevention, No.3, Likang Street, Shijiazhuang 050011, China
    These authors contributed equally to this work.)

  • Tian Qin

    (State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, No. 155, Changbai Road, Changping, Beijing 102206, China
    Collaborative Innovation Centre for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China
    These authors contributed equally to this work.)

  • Yun Li

    (Hebei International Travel Health-Care Center, No.409-1, Hongqi Stree, Shijiazhuang 050000, China)

  • Haijian Zhou

    (State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, No. 155, Changbai Road, Changping, Beijing 102206, China
    Collaborative Innovation Centre for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China)

  • Hongmei Song

    (Shijiazhuang Center for Disease Control and Prevention, No.3, Likang Street, Shijiazhuang 050011, China)

  • Hongyu Ren

    (State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, No. 155, Changbai Road, Changping, Beijing 102206, China
    Collaborative Innovation Centre for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China)

  • Liping Li

    (Shijiazhuang Center for Disease Control and Prevention, No.3, Likang Street, Shijiazhuang 050011, China)

  • Yongguang Li

    (Shijiazhuang Jiyuan Environmental Protection Technology Co.Ltd, Shijiazhuang 050035, China)

  • Dong Zhao

    (Shijiazhuang Center for Disease Control and Prevention, No.3, Likang Street, Shijiazhuang 050011, China)

Abstract

Cooling towers are a source of Legionnaires’ disease. It is important from a public health perspective to survey industrial cooling towers for the presence of Legionella . Prospective surveillance of the extent of Legionella pollution was conducted at factories in Shijiazhuang, China between March 2011 and September 2012. Overall, 35.7% of 255 industrial cooling tower water samples showed Legionella- positive, and their concentrations ranged from 100 Colony-Forming Units (CFU)/liter to 88,000 CFU/liter, with an average concentration of 9100 CFU/liter. A total of 121 isolates were obtained. All isolates were L. pneumophila , and the isolated serogroups included serogroups 1 (68 isolates, 56.2%), 6 (25, 20.7%), 5 (12, 9.9%), 8 (8, 6.6%), 3 (6, 5.0%) and 9 (2, 1.6%). All 121 isolates were analyzed by pulsed-field gel electrophoresis (PFGE) and 64 different patterns were obtained. All 121 isolates were analyzed sequence-based typing (SBT), a full 7-allele profile was obtained from 117 isolates. One hundred and seventeen isolates were divided into 49 sequence types. Two virulence genes, lvh and rtxA , are analyzed by polymerase chain reaction (PCR). 92.6% (112/121) and 98.3% (119/121) isolates carried lvh and rtxA respectively and 90.9% (110/121) of tested isolates carried both genes. Our results demonstrated high prevalence and genetic polymorphism of L. pneumophila in industrial cooling tower environments in Shijiazhang, China, and the SBT and virulence gene PCR results suggested that the isolates were pathogenic. Improved control and prevention strategies are urgently needed.

Suggested Citation

  • Lijie Li & Tian Qin & Yun Li & Haijian Zhou & Hongmei Song & Hongyu Ren & Liping Li & Yongguang Li & Dong Zhao, 2015. "Prevalence and Molecular Characteristics of Waterborne Pathogen Legionella in Industrial Cooling Tower Environments," IJERPH, MDPI, vol. 12(10), pages 1-13, October.
    • Handle: RePEc:gam:jijerp:v:12:y:2015:i:10:p:12605-12617:d:56966
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    Citations

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    Cited by:

    1. Noriko Nakanishi & Ryohei Nomoto & Shinobu Tanaka & Kentaro Arikawa & Tomotada Iwamoto, 2019. "Analysis of Genetic Characterization and Clonality of Legionella pneumophila Isolated from Cooling Towers in Japan," IJERPH, MDPI, vol. 16(9), pages 1-9, May.
    2. Leyi Zhang & Yi Li & Xin Wang & Zhihui Shangguan & Haijian Zhou & Yuejin Wu & Lianghuai Wang & Hongyu Ren & Yun Hu & Meifen Lin & Tian Qin, 2017. "High Prevalence and Genetic Polymorphisms of Legionella in Natural and Man-Made Aquatic Environments in Wenzhou, China," IJERPH, MDPI, vol. 14(3), pages 1-13, February.

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