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Protective Effect of Prolactin against Methylmercury-Induced Mutagenicity and Cytotoxicity on Human Lymphocytes

Author

Listed:
  • Liz Carmem Silva-Pereira

    (Federal Institute of Education, Science and Technology of Para, Itaituba Campus, IFPA Itaituba, Para 68180000, Brazil
    Biological Science Institute, Federal University of Para, Belem, Para 66075110, Brazil)

  • Carlos Alberto Machado Da Rocha

    (Education Federal Institute, Science and Technologie of Para, Belem Campus, IFPA. Belem, Para 66645240, Brazil)

  • Luiz Raimundo Campos da Silva e Cunha

    (Federal Institute of Education, Science and Technology of Para, Itaituba Campus, IFPA Itaituba, Para 68180000, Brazil)

  • Edmar Tavares Da Costa

    (Biological Science Institute, Federal University of Para, Belem, Para 66075110, Brazil)

  • Ana Paula Araújo Guimarães

    (Center of Biological and Health Sciences, University of Para, Belem Campus UEPA, Belem, Para 66050540, Brazil)

  • Thais Brilhante Pontes

    (Biological Science Institute, Federal University of Para, Belem, Para 66075110, Brazil)

  • Domingos Luiz Wanderley Picanço Diniz

    (Federal University of Western Para, Oriximiná Campus, UFOPA, Santarém, Para 68040470, Brazil)

  • Mariana Ferreira Leal

    (Department of Morphology and Genetics, Federal University of São Paulo, São Paulo 04021001 Brazil)

  • Caroline Aquino Moreira-Nunes

    (Biological Science Institute, Federal University of Para, Belem, Para 66075110, Brazil)

  • Rommel Rodríguez Burbano

    (Biological Science Institute, Federal University of Para, Belem, Para 66075110, Brazil)

Abstract

Mercury exhibits cytotoxic and mutagenic properties as a result of its effect on tubulin. This toxicity mechanism is related to the production of free radicals that can cause DNA damage. Methylmercury (MeHg) is one of the most toxic of the mercury compounds. It accumulates in the aquatic food chain, eventually reaching the human diet. Several studies have demonstrated that prolactin (PRL) may be differently affected by inorganic and organic mercury based on interference with various neurotransmitters involved in the regulation of PRL secretion. This study evaluated the cytoprotective effect of PRL on human lymphocytes exposed to MeHg in vitro , including observation of the kinetics of HL-60 cells (an acute myeloid leukemia lineage) treated with MeHg and PRL at different concentrations, with both treatments with the individual compounds and combined treatments. All treatments with MeHg produced a significant increase in the frequency of chromatid gaps, however, no significant difference was observed in the chromosomal breaks with any treatment. A dose-dependent increase in the mitotic index was observed for treatments with PRL, which also acts as a co-mitogenic factor, regulating proliferation by modulating the expression of genes that are essential for cell cycle progression and cytoskeleton organization. These properties contribute to the protective action of PRL against the cytotoxic and mutagenic effects of MeHg.

Suggested Citation

  • Liz Carmem Silva-Pereira & Carlos Alberto Machado Da Rocha & Luiz Raimundo Campos da Silva e Cunha & Edmar Tavares Da Costa & Ana Paula Araújo Guimarães & Thais Brilhante Pontes & Domingos Luiz Wander, 2014. "Protective Effect of Prolactin against Methylmercury-Induced Mutagenicity and Cytotoxicity on Human Lymphocytes," IJERPH, MDPI, vol. 11(9), pages 1-13, September.
    • Handle: RePEc:gam:jijerp:v:11:y:2014:i:9:p:9822-9834:d:40489
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