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Molecular Detection of Leptospiral DNA in Environmental Water on St. Kitts

Author

Listed:
  • Julienne Rawlins

    (Ross University School of Veterinary Medicine, Basseterre, St. Kitts, West Indies)

  • Alexandra Portanova

    (Ross University School of Veterinary Medicine, Basseterre, St. Kitts, West Indies)

  • Ilana Zuckerman

    (Ross University School of Veterinary Medicine, Basseterre, St. Kitts, West Indies)

  • Amanda Loftis

    (Ross University School of Veterinary Medicine, Basseterre, St. Kitts, West Indies
    Current address: Department of Microbiology & Immunology, Midwestern University, 19555 North 59th Avenue, Glendale, AZ 85308, USA)

  • Pietro Ceccato

    (International Research Institute for Climate and Society, The Earth Institute, Columbia University, Palisades, NY 10964, USA)

  • Arve Lee Willingham

    (Ross University School of Veterinary Medicine, Basseterre, St. Kitts, West Indies)

  • Ashutosh Verma

    (Ross University School of Veterinary Medicine, Basseterre, St. Kitts, West Indies)

Abstract

Leptospirosis is an important waterborne zoonotic disease caused by pathogenic Leptospira . The pathogen is maintained in a population due to chronic colonization and shedding from renal tubules of domestic and wild animals. Humans and other animals become infected when they come in contact with urine from infected animals, either directly or through urine-contaminated surface water. In this study, we screened environmental water on the island of St. Kitts by using a TaqMan based real time quantitative polymerase chain reaction (qPCR) targeting a pathogen specific leptospiral gene, lipl32 . Our results indicate that around one-fifth of tested water sources have detectable leptospiral DNA.

Suggested Citation

  • Julienne Rawlins & Alexandra Portanova & Ilana Zuckerman & Amanda Loftis & Pietro Ceccato & Arve Lee Willingham & Ashutosh Verma, 2014. "Molecular Detection of Leptospiral DNA in Environmental Water on St. Kitts," IJERPH, MDPI, vol. 11(8), pages 1-8, August.
    • Handle: RePEc:gam:jijerp:v:11:y:2014:i:8:p:7953-7960:d:38933
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    Cited by:

    1. Maria Cristina Schneider & Jorge Velasco-Hernandez & Kyung-duk Min & Deise Galan Leonel & David Baca-Carrasco & Matthew E. Gompper & Rudy Hartskeerl & Claudia Munoz-Zanzi, 2017. "The Use of Chemoprophylaxis after Floods to Reduce the Occurrence and Impact of Leptospirosis Outbreaks," IJERPH, MDPI, vol. 14(6), pages 1-18, June.

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