IDEAS home Printed from https://ideas.repec.org/a/gam/jijerp/v11y2014i11p11676-11690d42247.html
   My bibliography  Save this article

Liver δ-Aminolevulinate Dehydratase Activity is Inhibited by Neonicotinoids and Restored by Antioxidant Agents

Author

Listed:
  • Elisa Sauer

    (Laboratory of Toxicology (LATOX), Department of Clinical and Toxicology Analysis, Federal University of Rio Grande do Sul, Porto Alegre, RS 90610000, Brazil
    Institute of Cardiology, University Foundation of Cardiology, Porto Alegre, RS 90040371, Brazil)

  • Angela M. Moro

    (Laboratory of Toxicology (LATOX), Department of Clinical and Toxicology Analysis, Federal University of Rio Grande do Sul, Porto Alegre, RS 90610000, Brazil
    Laboratory of Toxicology, Center of Biological and Health Sciences University of Caxias do Sul, Caxias do Sul, RS, 96815900, Brazil
    Post-Graduate Program Pharmaceutical Sciences, Federal University of Rio Grande do Sul, Porto Alegre, RS 90610000, Brazil)

  • Natália Brucker

    (Laboratory of Toxicology (LATOX), Department of Clinical and Toxicology Analysis, Federal University of Rio Grande do Sul, Porto Alegre, RS 90610000, Brazil
    Post-Graduate Program Pharmaceutical Sciences, Federal University of Rio Grande do Sul, Porto Alegre, RS 90610000, Brazil
    Department of Clinical and Toxicology Analysis, Federal University of Santa Maria, RS 97105900, Brazil)

  • Sabrina Nascimento

    (Laboratory of Toxicology (LATOX), Department of Clinical and Toxicology Analysis, Federal University of Rio Grande do Sul, Porto Alegre, RS 90610000, Brazil
    Post-Graduate Program Pharmaceutical Sciences, Federal University of Rio Grande do Sul, Porto Alegre, RS 90610000, Brazil)

  • Bruna Gauer

    (Laboratory of Toxicology (LATOX), Department of Clinical and Toxicology Analysis, Federal University of Rio Grande do Sul, Porto Alegre, RS 90610000, Brazil)

  • Rafael Fracasso

    (Laboratory of Toxicology (LATOX), Department of Clinical and Toxicology Analysis, Federal University of Rio Grande do Sul, Porto Alegre, RS 90610000, Brazil
    Post-Graduate Program Pharmaceutical Sciences, Federal University of Rio Grande do Sul, Porto Alegre, RS 90610000, Brazil)

  • Adriana Gioda

    (Department of Chemistry, Pontifical Catholic University of Rio de Janeiro (PUC-Rio), Rio de Janeiro, RJ 22453900, Brazil)

  • Ruy Beck

    (Department of Production and Drug Control, Federal University of Rio Grande do Sul, Porto Alegre, RS 90610000, Brazil)

  • José C. F. Moreira

    (Department of Biochemistry, Federal University of Rio Grande do Sul, Porto Alegre, RS 90035003, Brazil)

  • Vera Lucia Eifler-Lima

    (Department of Production of Source-Material, Federal University of Rio Grande do Sul, Porto Alegre, RS 90610000, Brazil)

  • Solange Cristina Garcia

    (Laboratory of Toxicology (LATOX), Department of Clinical and Toxicology Analysis, Federal University of Rio Grande do Sul, Porto Alegre, RS 90610000, Brazil
    Institute of Cardiology, University Foundation of Cardiology, Porto Alegre, RS 90040371, Brazil
    Department of Clinical and Toxicology Analysis, Federal University of Santa Maria, RS 97105900, Brazil)

Abstract

Neonicotinoids represent the most used class of insecticides worldwide, and their precursor, imidacloprid, is the most widely marketed. The aim of this study was to evaluate the effect of imidacloprid on the activity of hepatic δ-aminolevulinate dehydratase (δ-ALA-D), protective effect of potential antioxidants against this potential effect and presence of chemical elements in the constitution of this pesticide. We observed that δ-ALA-D activity was significantly inhibited by imidacloprid at all concentrations tested in a dose-dependent manner. The IC 50 value was obtained and used to evaluate the restoration of the enzymatic activity. δ-ALA-D inhibition was completely restored by addition of dithiotreitol (DTT) and partly by ZnCl 2, demonstrating that the inhibition occurs by oxidation of thiol groups and by displacement of the Zn (II), which can be explained by the presence of chemical elements found in the constitution of pesticides. Reduced glutathione (GSH) had the best antioxidant effect against to δ-ALA-D inhibition caused by imidacloprid, followed by curcumin and resveratrol. It is well known that inhibition of the enzyme δ-ALA-D may result in accumulation of its neurotoxic substrate (δ-ALA), in this line, our results suggest that further studies are needed to investigate the possible neurotoxicity induced by neonicotinoids and the involvement of antioxidants in cases of poisoning by neonicotinoids.

Suggested Citation

  • Elisa Sauer & Angela M. Moro & Natália Brucker & Sabrina Nascimento & Bruna Gauer & Rafael Fracasso & Adriana Gioda & Ruy Beck & José C. F. Moreira & Vera Lucia Eifler-Lima & Solange Cristina Garcia, 2014. "Liver δ-Aminolevulinate Dehydratase Activity is Inhibited by Neonicotinoids and Restored by Antioxidant Agents," IJERPH, MDPI, vol. 11(11), pages 1-15, November.
    • Handle: RePEc:gam:jijerp:v:11:y:2014:i:11:p:11676-11690:d:42247
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1660-4601/11/11/11676/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1660-4601/11/11/11676/
    Download Restriction: no
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Leidiane De Lucca & Fabiane Rodrigues & Letícia B. Jantsch & Walter S. Neme & Francisco M. P. Gallarreta & Thissiane L. Gonçalves, 2016. "Oxidative Profile and δ-Aminolevulinate Dehydratase Activity in Healthy Pregnant Women with Iron Supplementation," IJERPH, MDPI, vol. 13(5), pages 1-10, May.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jijerp:v:11:y:2014:i:11:p:11676-11690:d:42247. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.