IDEAS home Printed from https://ideas.repec.org/a/gam/jijerp/v11y2014i11p11421-11437d41995.html
   My bibliography  Save this article

Multivariate Optimization for Extraction of Pyrethroids in Milk and Validation for GC-ECD and CG-MS/MS Analysis

Author

Listed:
  • Leonardo Zanchetti Meneghini

    (Laboratório Nacional Agropecuário, Ministério da Agricultura, Pecuária e Abastecimento, Estrada da Ponta Grossa, 3036 Porto Alegre, RS 91785-340, Brazil
    Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga, 2752 Porto Alegre, RS 90610-000, Brazil)

  • Gabriel Rübensam

    (Laboratório Nacional Agropecuário, Ministério da Agricultura, Pecuária e Abastecimento, Estrada da Ponta Grossa, 3036 Porto Alegre, RS 91785-340, Brazil)

  • Vinicius Claudino Bica

    (Laboratório Nacional Agropecuário, Ministério da Agricultura, Pecuária e Abastecimento, Estrada da Ponta Grossa, 3036 Porto Alegre, RS 91785-340, Brazil)

  • Amanda Ceccon

    (Laboratório Nacional Agropecuário, Ministério da Agricultura, Pecuária e Abastecimento, Estrada da Ponta Grossa, 3036 Porto Alegre, RS 91785-340, Brazil
    Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga, 2752 Porto Alegre, RS 90610-000, Brazil)

  • Fabiano Barreto

    (Laboratório Nacional Agropecuário, Ministério da Agricultura, Pecuária e Abastecimento, Estrada da Ponta Grossa, 3036 Porto Alegre, RS 91785-340, Brazil)

  • Marco Flores Ferrão

    (Instituto de Química, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500 Bairro Agronomia, Porto Alegre, RS 91501-970, Brazil)

  • Ana Maria Bergold

    (Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga, 2752 Porto Alegre, RS 90610-000, Brazil)

Abstract

A simple and inexpensive method based on solvent extraction followed by low temperature clean-up was applied for determination of seven pyrethroids residues in bovine raw milk using gas chromatography coupled to tandem mass spectrometry (GC-MS/MS) and gas chromatography with electron-capture detector (GC-ECD). Sample extraction procedure was established through the evaluation of seven different extraction protocols, evaluated in terms of analyte recovery and cleanup efficiency. Sample preparation optimization was based on Doehlert design using fifteen runs with three different variables. Response surface methodologies and polynomial analysis were used to define the best extraction conditions. Method validation was carried out based on SANCO guide parameters and assessed by multivariate analysis. Method performance was considered satisfactory since mean recoveries were between 87% and 101% for three distinct concentrations. Accuracy and precision were lower than ±20%, and led to no significant differences ( p < 0.05) between results obtained by GC-ECD and GC-MS/MS techniques. The method has been applied to routine analysis for determination of pyrethroid residues in bovine raw milk in the Brazilian National Residue Control Plan since 2013, in which a total of 50 samples were analyzed.

Suggested Citation

  • Leonardo Zanchetti Meneghini & Gabriel Rübensam & Vinicius Claudino Bica & Amanda Ceccon & Fabiano Barreto & Marco Flores Ferrão & Ana Maria Bergold, 2014. "Multivariate Optimization for Extraction of Pyrethroids in Milk and Validation for GC-ECD and CG-MS/MS Analysis," IJERPH, MDPI, vol. 11(11), pages 1-17, November.
    • Handle: RePEc:gam:jijerp:v:11:y:2014:i:11:p:11421-11437:d:41995
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1660-4601/11/11/11421/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1660-4601/11/11/11421/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Camilo Lesmes-Fabian & Claudia R. Binder, 2013. "Pesticide Flow Analysis to Assess Human Exposure in Greenhouse Flower Production in Colombia," IJERPH, MDPI, vol. 10(4), pages 1-18, March.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Camilo Lesmes Fabian & Claudia R. Binder, 2015. "Dermal Exposure Assessment to Pesticides in Farming Systems in Developing Countries: Comparison of Models," IJERPH, MDPI, vol. 12(5), pages 1-27, April.
    2. Meaza Gezu Shentema & Magne Bråtveit & Abera Kumie & Wakgari Deressa & Bente Elisabeth Moen, 2022. "Respiratory Health among Pesticide Sprayers at Flower Farms in Ethiopia," IJERPH, MDPI, vol. 19(12), pages 1-9, June.
    3. Meaza Gezu Shentema & Abera Kumie & Magne Bråtveit & Wakgari Deressa & Aiwerasia Vera Ngowi & Bente E. Moen, 2020. "Pesticide Use and Serum Acetylcholinesterase Levels among Flower Farm Workers in Ethiopia—A Cross-Sectional Study," IJERPH, MDPI, vol. 17(3), pages 1-14, February.
    4. Suphaphat Kwonpongsagoon & Chanokwan Katasila & Pornpimol Kongtip & Susan Woskie, 2021. "Application Intensity and Spatial Distribution of Three Major Herbicides from Agricultural and Nonagricultural Practices in the Central Plain of Thailand," IJERPH, MDPI, vol. 18(6), pages 1-19, March.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jijerp:v:11:y:2014:i:11:p:11421-11437:d:41995. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.