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A Novel Miniature Culture System to Screen CO 2 -Sequestering Microalgae

Author

Listed:
  • Wei Han

    (School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China)

  • Chunying Li

    (School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China)

  • Xiaoling Miao

    (School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
    State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, China)

  • Guangxin Yu

    (New Energy Investment Co. Ltd., Beijing 100016, China)

Abstract

In this study, a novel 96-well microplate swivel system (M96SS) was built for high-throughput screening of microalgal strains for CO 2 fixation. Cell growth under different CO 2 supply conditions (0.2, 0.4, 0.8, and 1.2 g L −1 d −1 ), residual nitrate, and pH value of Chlorella sp. SJTU-3, Chlorella pyrenoidosa SJTU-2, and Scenedesmus obliquus SJTU-3 were examined in the M96SS and traditional flask cultures. The dynamic data showed there was a good agreement between the systems. Two critical problems in miniature culture systems (intra-well mixing and evaporation loss) were improved by sealed vertical mixing of the M96SS. A sample screen of six microalgal species ( Chlorella sp. SJTU-3, Chlorella pyrenoidosa SJTU-2, Selenastrum capricornutum , Scenedesmus obliquus SJTU-3, Chlamydomonas sajao , Dunaliella primolecta ) was carried out in flasks and the M96SS. Chlamydomonas sajao appeared to be a robust performer (highest cell density: 1.437 g L −1 ) in anaerobic pond water with 0.8, and 1.2 g L −1 d −1 CO 2 . The reliability and efficiency of the M96SS were verified through a comparison of traditional flask culture, M96SS, Lukavský’s system, and a microplate shaker.

Suggested Citation

  • Wei Han & Chunying Li & Xiaoling Miao & Guangxin Yu, 2012. "A Novel Miniature Culture System to Screen CO 2 -Sequestering Microalgae," Energies, MDPI, vol. 5(11), pages 1-18, November.
    • Handle: RePEc:gam:jeners:v:5:y:2012:i:11:p:4372-4389:d:21122
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    Cited by:

    1. Zhou, Wenguang & Wang, Jinghan & Chen, Paul & Ji, Chengcheng & Kang, Qiuyun & Lu, Bei & Li, Kun & Liu, Jin & Ruan, Roger, 2017. "Bio-mitigation of carbon dioxide using microalgal systems: Advances and perspectives," Renewable and Sustainable Energy Reviews, Elsevier, vol. 76(C), pages 1163-1175.
    2. Olia, Mahroo Seyed Jafari & Azin, Mehrdad & Sepahi, Abbas Akhavan & Moazami, Nasrin, 2020. "Miniaturized culture method for the statistical study of growth rate and carbohydrate content of Picochlorum sp. D8 isolated from the Persian Gulf," Renewable Energy, Elsevier, vol. 149(C), pages 479-488.

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