Author
Listed:
- Sajag Adhikari
(Department of Agronomy, Horticulture and Plant Science, South Dakota State University, Brookings, SD 57007, USA
Department of Agronomy and Horticulture, University of Nebraska, Lincoln, NE 68583, USA)
- Suresh Damodaran
(Department of Agronomy, Horticulture and Plant Science, South Dakota State University, Brookings, SD 57007, USA
Department of Biology, Washington University in St Louis, St Louis, MO 63130, USA)
- Senthil Subramanian
(Department of Agronomy, Horticulture and Plant Science, South Dakota State University, Brookings, SD 57007, USA
Department of Biology & Microbiology, South Dakota State University, Brookings, SD 57007, USA)
Abstract
Symbiotic legume nodules and lateral roots arise away from the root meristem via dedifferentiation events. While these organs share some morphological and developmental similarities, whether legume nodules are modified lateral roots is an open question. We dissected emerging nodules, mature nodules, emerging lateral roots and young lateral roots, and constructed strand-specific RNA sequencing (RNAseq) libraries using polyA-enriched RNA preparations. Root sections above and below these organs, devoid of any lateral organs, were used to construct respective control tissue libraries. High sequence quality, predominant mapping to coding sequences, and consistency between replicates indicated that the RNAseq libraries were of a very high quality. We identified genes enriched in emerging nodules, mature nodules, emerging lateral roots and young lateral roots in soybean by comparing global gene expression profiles between each of these organs and adjacent root segments. Potential uses for this high quality transcriptome data set include generation of global gene regulatory networks to identify key regulators; metabolic pathway analyses and comparative analyses of key gene families to discover organ-specific biological processes; and identification of organ-specific alternate spliced transcripts. When combined with other similar datasets, especially from leguminous plants, these analyses can help answer questions on the evolutionary origins of root nodules and relationships between the development of different plant lateral organs.
Suggested Citation
Sajag Adhikari & Suresh Damodaran & Senthil Subramanian, 2019.
"Lateral Root and Nodule Transcriptomes of Soybean,"
Data, MDPI, vol. 4(2), pages 1-11, May.
Handle:
RePEc:gam:jdataj:v:4:y:2019:i:2:p:64-:d:229214
Download full text from publisher
Corrections
All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jdataj:v:4:y:2019:i:2:p:64-:d:229214. See general information about how to correct material in RePEc.
If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.
We have no bibliographic references for this item. You can help adding them by using this form .
If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.
For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .
Please note that corrections may take a couple of weeks to filter through
the various RePEc services.