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ARTD10/PARP10 Induces ADP-Ribosylation of GAPDH and Recruits GAPDH into Cytosolic Membrane-Free Cell Bodies When Overexpressed in Mammalian Cells

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  • Emilia Mayo

    (G-Protein-mediated Signalling Laboratory, and Signal-Dependent Transcription Laboratory, Fondazione Mario Negri Sud, Via Nazionale, 8/A, 66030 Santa Maria Imbaro (CH), Italy
    Present Address: E.M.: Dompè, Via Castellino 111, 80131 Napoli, Italy. N.D.: Bioclarma srl, Via Nizza 52, 10126 Torino, Italy. F.C.: European Institute of Oncology, Via Adamello 16, 20139 Milano, Italy.)

  • Gaia Fabrizio

    (G-Protein-mediated Signalling Laboratory, and Signal-Dependent Transcription Laboratory, Fondazione Mario Negri Sud, Via Nazionale, 8/A, 66030 Santa Maria Imbaro (CH), Italy)

  • Emanuele Salvatore Scarpa

    (G-Protein-mediated Signalling Laboratory, and Signal-Dependent Transcription Laboratory, Fondazione Mario Negri Sud, Via Nazionale, 8/A, 66030 Santa Maria Imbaro (CH), Italy)

  • Annalisa Stilla

    (G-Protein-mediated Signalling Laboratory, and Signal-Dependent Transcription Laboratory, Fondazione Mario Negri Sud, Via Nazionale, 8/A, 66030 Santa Maria Imbaro (CH), Italy)

  • Nadia Dani

    (G-Protein-mediated Signalling Laboratory, and Signal-Dependent Transcription Laboratory, Fondazione Mario Negri Sud, Via Nazionale, 8/A, 66030 Santa Maria Imbaro (CH), Italy
    Present Address: E.M.: Dompè, Via Castellino 111, 80131 Napoli, Italy. N.D.: Bioclarma srl, Via Nizza 52, 10126 Torino, Italy. F.C.: European Institute of Oncology, Via Adamello 16, 20139 Milano, Italy.)

  • Fulvio Chiacchiera

    (G-Protein-mediated Signalling Laboratory, and Signal-Dependent Transcription Laboratory, Fondazione Mario Negri Sud, Via Nazionale, 8/A, 66030 Santa Maria Imbaro (CH), Italy
    Present Address: E.M.: Dompè, Via Castellino 111, 80131 Napoli, Italy. N.D.: Bioclarma srl, Via Nizza 52, 10126 Torino, Italy. F.C.: European Institute of Oncology, Via Adamello 16, 20139 Milano, Italy.)

  • Henning Kleine

    (Institute of Biochemistry and Molecular Biology, Medical School, RWTH Aachen University, Pauwelsstraße 30, 52074 Aachen, Germany)

  • Francesca Attanasio

    (G-Protein-mediated Signalling Laboratory, and Signal-Dependent Transcription Laboratory, Fondazione Mario Negri Sud, Via Nazionale, 8/A, 66030 Santa Maria Imbaro (CH), Italy)

  • Bernhard Lüscher

    (Institute of Biochemistry and Molecular Biology, Medical School, RWTH Aachen University, Pauwelsstraße 30, 52074 Aachen, Germany)

  • Maria Di Girolamo

    (G-Protein-mediated Signalling Laboratory, and Signal-Dependent Transcription Laboratory, Fondazione Mario Negri Sud, Via Nazionale, 8/A, 66030 Santa Maria Imbaro (CH), Italy)

Abstract

Protein ADP-ribosylation is a reversible post-translational modification of cellular proteins that is catalysed by enzymes that transfer one (mono) or several (poly) units of ADP-ribose from β-NAD + to a specific amino acid of the target protein. The most studied member of the ADP-ribosyltransferase family is PARP1 (also known as ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1), which is directly activated by DNA strand breaks and is involved in DNA damage repair, chromatin remodelling and transcriptional regulation. Much less is known about the further 16 members of this family. Among these, ARTD10/PARP10 has been previously characterised as a mono-ADP-ribosyltransferase with a role in cell proliferation and in NF-kB signalling. In the present study, we identified the glycolytic enzyme GAPDH as an interactor and a novel cellular target for ARTD10/PARP10. Moreover, we detected the co-localisation of GAPDH and ARTD10/PARP10 in well-defined cytosolic bodies, which we show here to be membrane-free, rounded structures using immunogold labelling and electron microscopy. Using the cognitive binding module macro domain to visualise ADP-ribosylated proteins by immunofluorescence microscopy in cells over-expressing the ARTD10/PARP10 enzyme, we show that the staining of the ARTD10/PARP10-dependent cytosolic bodies was lost when the cells were treated with compounds that inhibit ARTD10/PARP10, either by directly inhibiting the enzyme or by reducing the cellular NAD + levels. In parallel, the same treatment affected the co-localisation of GAPDH and ARTD10/PARP10, as GAPDH disappeared from the cytosolic cell bodies, which indicates that its presence there depends on the catalytic activity of ARTD10/PARP10. In line with this, in cells over-expressing the ARTD10/PARP10 catalytic domain alone, which we show here to form stress granules, GAPDH was recruited into stress granules. These data identify ARTD10/PARP10 as the enzyme that modifies and recruits GAPDH into cytosolic structures.

Suggested Citation

  • Emilia Mayo & Gaia Fabrizio & Emanuele Salvatore Scarpa & Annalisa Stilla & Nadia Dani & Fulvio Chiacchiera & Henning Kleine & Francesca Attanasio & Bernhard Lüscher & Maria Di Girolamo, 2018. "ARTD10/PARP10 Induces ADP-Ribosylation of GAPDH and Recruits GAPDH into Cytosolic Membrane-Free Cell Bodies When Overexpressed in Mammalian Cells," Challenges, MDPI, vol. 9(1), pages 1-19, May.
  • Handle: RePEc:gam:jchals:v:9:y:2018:i:1:p:22-:d:144416
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    References listed on IDEAS

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    1. Patricia Verheugd & Alexandra H. Forst & Larissa Milke & Nicolas Herzog & Karla L.H. Feijs & Elisabeth Kremmer & Henning Kleine & Bernhard Lüscher, 2013. "Regulation of NF-κB signalling by the mono-ADP-ribosyltransferase ARTD10," Nature Communications, Nature, vol. 4(1), pages 1-11, June.
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    Cited by:

    1. Maria Di Girolamo & Gaia Fabrizio, 2018. "The ADP-Ribosyl-Transferases Diphtheria Toxin-Like (ARTDs) Family: An Overview," Challenges, MDPI, vol. 9(1), pages 1-24, May.

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    1. Maria Di Girolamo & Gaia Fabrizio, 2018. "The ADP-Ribosyl-Transferases Diphtheria Toxin-Like (ARTDs) Family: An Overview," Challenges, MDPI, vol. 9(1), pages 1-24, May.

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