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Microarray-Based Screening of Differentially Expressed Genes of E. coli O157:H7 Sakai during Preharvest Survival on Butterhead Lettuce

Author

Listed:
  • Inge Van der Linden

    (Institute for Agricultural and Fisheries Research (ILVO), Plant Sciences Unit—Crop Protection B. Van Gansberghelaan 96, Merelbeke B-9820, Belgium
    Institute for Agricultural and Fisheries Research (ILVO), Food Sciences Unit—Food Safety Brusselsesteenweg 370, Melle B-9090, Belgium
    Ghent University (UGent), Department of Food Safety and Food Quality, Laboratory of Food Microbiology and Food Preservation, Coupure links 653, Ghent B-9000, Belgium)

  • Bart Cottyn

    (Institute for Agricultural and Fisheries Research (ILVO), Plant Sciences Unit—Crop Protection B. Van Gansberghelaan 96, Merelbeke B-9820, Belgium)

  • Mieke Uyttendaele

    (Ghent University (UGent), Department of Food Safety and Food Quality, Laboratory of Food Microbiology and Food Preservation, Coupure links 653, Ghent B-9000, Belgium)

  • Geertrui Vlaemynck

    (Institute for Agricultural and Fisheries Research (ILVO), Food Sciences Unit—Food Safety Brusselsesteenweg 370, Melle B-9090, Belgium)

  • Marc Heyndrickx

    (Institute for Agricultural and Fisheries Research (ILVO), Food Sciences Unit—Food Safety Brusselsesteenweg 370, Melle B-9090, Belgium
    Ghent University (UGent), Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Sciences, Salisburylaan 133, Merelbeke B-9820, Belgium)

  • Martine Maes

    (Institute for Agricultural and Fisheries Research (ILVO), Plant Sciences Unit—Crop Protection B. Van Gansberghelaan 96, Merelbeke B-9820, Belgium
    Ghent University (UGent), Department of Biochemistry and Microbiology, Laboratory of Microbiology, K.L. Ledeganckstraat 35, Ghent B-9000, Belgium)

  • Nicola Holden

    (The James Hutton Institute, Cell and Molecular Sciences, Human and Animal Pathogens on Plants, Invergowrie, Dundee DD2 5DA, UK)

Abstract

Numerous outbreaks of Escherichia coli O157:H7 have been linked to the consumption of leafy vegetables. However, up to the present, little has been known about E. coli O157:H7’s adaptive responses to survival on actively growing (and thus responsive) plants. In this study, whole genome transcriptional profiles were generated from E. coli O157:H7 cells (isolate Sakai, stx -) one hour and two days after inoculation on the leaves of growing butterhead lettuce, and compared with an inoculum control. A total of 273 genes of E. coli O157:H7 Sakai (5.04% of the whole genome) were significantly induced or repressed by at least two-fold ( p < 0.01) in at least one of the analyzed time points in comparison with the control. Several E. coli O157:H7 genes associated with oxidative stress and antimicrobial resistance were upregulated, including the iron-sulfur cluster and the multiple antibiotic resistance ( mar ) operon, whereas the Shiga toxin virulence genes were downregulated. Nearly 40% of the genes with significantly different expression were poorly characterized genes or genes with unknown functions. These genes are of special interest for future research as they may play an important role in the pathogens’ adaptation to a lifestyle on plants. In conclusion, these findings suggest that the pathogen actively interacts with the plant environment by adapting its metabolism and responding to oxidative stress.

Suggested Citation

  • Inge Van der Linden & Bart Cottyn & Mieke Uyttendaele & Geertrui Vlaemynck & Marc Heyndrickx & Martine Maes & Nicola Holden, 2016. "Microarray-Based Screening of Differentially Expressed Genes of E. coli O157:H7 Sakai during Preharvest Survival on Butterhead Lettuce," Agriculture, MDPI, vol. 6(1), pages 1-22, January.
    • Handle: RePEc:gam:jagris:v:6:y:2016:i:1:p:6-:d:62922
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    1. anonymous, 2012. "Noteworthy: Texas tourism, banking, agriculture," Southwest Economy, Federal Reserve Bank of Dallas, issue Q2, pages 1-14.
    2. Haluk Duman & Rabia Ozpeynirci & M. Yilmaz Icerli, 2012. "Agricultural Activities – TAS 41: Turkey Example," International Journal of Academic Research in Accounting, Finance and Management Sciences, Human Resource Management Academic Research Society, International Journal of Academic Research in Accounting, Finance and Management Sciences, vol. 2(3), pages 118-131, July.
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