IDEAS home Printed from https://ideas.repec.org/a/gam/jagris/v15y2025i4p363-d1586703.html
   My bibliography  Save this article

Techniques for In Vitro Fertilisation of Vitrified Cattle Oocytes: Challenges and New Developments

Author

Listed:
  • Mahlatsana Ramaesela Ledwaba

    (Agricultural Research Council, Animal Production, Germplasm Conservation and Reproductive Biotechnologies, Private Bag X2, Pretoria 0062, South Africa
    Department of Animal, Wildlife and Grassland Sciences, University of the Free State, P.O. Box 339, Bloemfontein 9300, South Africa)

  • Hester Adri O’Neill

    (Department of Animal, Wildlife and Grassland Sciences, University of the Free State, P.O. Box 339, Bloemfontein 9300, South Africa)

  • Mamonene Angelinah Thema

    (Agricultural Research Council, Animal Production, Germplasm Conservation and Reproductive Biotechnologies, Private Bag X2, Pretoria 0062, South Africa)

  • Ayanda Maqhashu

    (Department of Animal, Wildlife and Grassland Sciences, University of the Free State, P.O. Box 339, Bloemfontein 9300, South Africa)

  • Masindi Lottus Mphaphathi

    (Agricultural Research Council, Animal Production, Germplasm Conservation and Reproductive Biotechnologies, Private Bag X2, Pretoria 0062, South Africa)

Abstract

Cryopreservation is currently an essential technique in reproductive technologies that is used for the extended preservation of genetic material. Vitrification has become the industry’s standard cryopreservation technique for cattle oocytes and embryos. The current results of this technology, however, are still not good in terms of viability, fertilisation capacity, embryo development, or pregnancy. The oocytes’ susceptibility to freezing is associated with significant changes in the structures, functioning of the oocytes, and cryoinjury, which is harmful to the survival of cells and their subsequent growth. The effectiveness of producing embryos with in vitro techniques utilising vitrified cattle oocytes rarely exceeds 30–40%. A significant number of vitrified oocytes do not successfully develop into the embryo stage following in vitro fertilisation and culture. This review focuses on issues related to oocyte cryopreservation, ways to overcome them, and how to enhance the vitrified oocyte fertilisation process.

Suggested Citation

  • Mahlatsana Ramaesela Ledwaba & Hester Adri O’Neill & Mamonene Angelinah Thema & Ayanda Maqhashu & Masindi Lottus Mphaphathi, 2025. "Techniques for In Vitro Fertilisation of Vitrified Cattle Oocytes: Challenges and New Developments," Agriculture, MDPI, vol. 15(4), pages 1-20, February.
    • Handle: RePEc:gam:jagris:v:15:y:2025:i:4:p:363-:d:1586703
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2077-0472/15/4/363/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2077-0472/15/4/363/
    Download Restriction: no
    ---><---

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jagris:v:15:y:2025:i:4:p:363-:d:1586703. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.