IDEAS home Printed from https://ideas.repec.org/a/gam/jagris/v14y2024i5p698-d1385438.html
   My bibliography  Save this article

Isolation, Characterization, and Biopreservation of Lactobacillus brevis DN-1 to Inhibit Mold and Remove Aflatoxin B1 in Peanut and Sunflower Cakes

Author

Listed:
  • Xiaoni Wang

    (College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao 028000, China)

  • Siyuan Wang

    (College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao 028000, China)

  • Junzhao Xu

    (College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao 028000, China)

  • Baiyila Wu

    (College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao 028000, China)

  • Zongfu Hu

    (College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao 028000, China)

  • Huaxin Niu

    (College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao 028000, China)

Abstract

Aflatoxin B1 (AFB1) is the most toxic mycotoxin and is widespread in moldy feed. The use of biological removal methods to reduce AFB1 has become a research hotspot. This study aimed to isolate lactic acid bacteria (LAB) capable of removing AFB1 from moldy feeds and assessed the removal capacity under various environmental conditions. A strain named Lactobacillus brevis DN-1 was isolated from feed samples and showed 71.38% AFB1 percent removal. Furthermore, DN-1 showed good antifungal activity against Aspergillus flavus BNCC336156 and Aspergillus parasiticus BNCC335939. The optimum growth temperature and pH of DN-1 were 37 °C and 6.0, respectively, and DN-1 grew well in the concentration range of 0–20 µg/L AFB1. Under a temperature of 20–40 °C, pH of 3.0–9.0, and anaerobic conditions, the percent removal of AFB1 was more than 60%. An analysis of the different components of DN-1 showed that cell wall adsorption was the main removal method and suggested the pathway for AFB1 removal by LAB. In addition, strain DN-1 was used as a biological preservative in artificially contaminated peanut and sunflower cakes, which significantly inhibited the growth of mold and production of AFB1. In brief, this study highlights the potential use of DN-1 as a preventive agent against aflatoxicosis via strong removal capability in the application of fermented feed or food.

Suggested Citation

  • Xiaoni Wang & Siyuan Wang & Junzhao Xu & Baiyila Wu & Zongfu Hu & Huaxin Niu, 2024. "Isolation, Characterization, and Biopreservation of Lactobacillus brevis DN-1 to Inhibit Mold and Remove Aflatoxin B1 in Peanut and Sunflower Cakes," Agriculture, MDPI, vol. 14(5), pages 1-13, April.
    • Handle: RePEc:gam:jagris:v:14:y:2024:i:5:p:698-:d:1385438
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2077-0472/14/5/698/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2077-0472/14/5/698/
    Download Restriction: no
    ---><---

    More about this item

    Keywords

    aflatoxin B1; Lactobacillus brevis DN-1; removal; mycotoxin; feed or food safety;
    All these keywords.

    JEL classification:

    • B1 - Schools of Economic Thought and Methodology - - History of Economic Thought through 1925

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jagris:v:14:y:2024:i:5:p:698-:d:1385438. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.