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A Genome-Wide Comparative Analysis of AUX1/LAX , PIN, and ABCB Genes Reveals Their Roles in Cucumber Fruit Curving

Author

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  • Ke Lu

    (College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080, China
    Key Laboratory of Sugar Beet Genetic Breeding, Heilongjiang University, Harbin 150080, China)

  • La Zhang

    (College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080, China
    Key Laboratory of Sugar Beet Genetic Breeding, Heilongjiang University, Harbin 150080, China)

  • Lianxue Fan

    (Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture, College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China)

  • Xiuyan Zhou

    (Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture, College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China)

  • Shengnan Li

    (College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080, China
    Key Laboratory of Sugar Beet Genetic Breeding, Heilongjiang University, Harbin 150080, China)

Abstract

Auxin transport is regulated by the AUX1/LAX , PIN , and ABCB gene families, controlling the distribution of auxin and ultimately fruit curving in cucumbers. However, studies on the differential expression of these auxin transporters and their roles in fruit curving are limited. In this study, we identified 36 auxin transporters from cucumber, including CsLAX1–7, CsPIN1–10, and CsABCB1–19. Basic characteristic analysis revealed that all CsLAX proteins were conservative, and a C-terminal NPNTY motif was found in CsPIN1–4/7–10. CsABCB1/5/11/14/17 were categorized as half-size transporters. Phylogenetic analysis revealed a genetic relationship between auxin transporters in Arabidopsis and cucumber. Exogenous auxin treatment on fruits and qPCR analysis indicated that differential expression patterns of auxin transporters control cucumber fruit curving. Co-expression analysis indicated that CsPIN1 and CsLAX2 were substantially negatively correlated, and they displayed opposite expression patterns in curved fruits. A proposed model suggested that CsLAX2 transports extracellular auxin to the convex side of the fruit; however, CsPIN1 inhibits auxin efflux at the same location. This leads to uneven auxin distribution that results in cucumber fruit curving.

Suggested Citation

  • Ke Lu & La Zhang & Lianxue Fan & Xiuyan Zhou & Shengnan Li, 2024. "A Genome-Wide Comparative Analysis of AUX1/LAX , PIN, and ABCB Genes Reveals Their Roles in Cucumber Fruit Curving," Agriculture, MDPI, vol. 14(5), pages 1-17, April.
    • Handle: RePEc:gam:jagris:v:14:y:2024:i:5:p:657-:d:1381807
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    References listed on IDEAS

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    1. Zhisen Yang & Jing Xia & Jingjing Hong & Chenxi Zhang & Hong Wei & Wei Ying & Chunqiao Sun & Lianghanxiao Sun & Yanbo Mao & Yongxiang Gao & Shutang Tan & Jiří Friml & Dianfan Li & Xin Liu & Linfeng Su, 2022. "Structural insights into auxin recognition and efflux by Arabidopsis PIN1," Nature, Nature, vol. 609(7927), pages 611-615, September.
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