IDEAS home Printed from https://ideas.repec.org/a/gam/jagris/v13y2023i5p1041-d1144579.html
   My bibliography  Save this article

Analysis of the Biological Activity and Whole Genome Sequencing of Bacillus cereus CDHWZ7 Isolated from the Rhizosphere of Lycium ruthenicum on the Tibetan Plateau

Author

Listed:
  • Xue Yang

    (College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China
    Key Laboratory of Use of Forage Germplasm Resources on Tibetan Plateau of Qinghai Province, Xining 810016, China)

  • Yongli Xie

    (College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China
    Key Laboratory of Use of Forage Germplasm Resources on Tibetan Plateau of Qinghai Province, Xining 810016, China
    State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, China)

  • Youming Qiao

    (State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, China)

  • Lan Chen

    (College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China)

  • Tian Wang

    (College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China)

  • Lingling Wu

    (College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China)

  • Junxi Li

    (College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China)

  • Ying Gao

    (College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China)

Abstract

This study examined the biological activity and genome of Bacillus cereus CDHWZ7 isolated from the root of Lycium ruthenicum in the Dachaidan saline area, Haixi Prefecture, Qinghai Province, China. The results revealed that B. cereus CDHWZ7 exhibited strong inhibition activity against the pathogenic fungi Fusarium graminearum , F. acuminatum , and F. oxysporum . CDHWZ7 also demonstrated cellulose-degrading activity, nitrogen-fixing activity, and the ability to secrete indole-3-acetic acid (IAA) at 55.00 mg∙L −1 . The strain CDHWZ7 can grow at a salt concentration of 3–11%, a pH range of 5–11, and a temperature of 4 °C–18 °C, and shows good salt tolerance, acid and alkaline tolerance, and low-temperature fitness. The genome of strain CDHWZ7 was sequenced using Illumina HiSeq + PacBio, revealing a circular structure of 5,648,783 bp in length, containing two intact plasmids with an average GC content of 35.2%, and a total number of 5672 encoded genes. It contained 106 tRNA genes, 42 rRNA genes, and 134 sRNA genes. A total of 137 genes were annotated as carbohydrases, with a total base length of 3,968,396,297 bp. The numbers of coding sequences assigned to the Kyoto Encyclopedia of Genes and Genomes, Clusters of Orthologous Groups of Proteins, and Gene Ontology Databases were 4038, 4133, and 2160, respectively. Further analysis of the genome identified genes encoding chitinase activity, cellulases, secondary metabolites, phytohormone production, volatile compounds, nitrogen and phosphate metabolism, and resistance responses to biotic stresses (glycine betaine transporter protein, catalase, superoxide dismutase, low-affinity potassium transporter protein, cold-shock protein, heat-shock protein), as well as genes related to proliferation, stress response, and resistance to pathogenic fungi. Therefore, this study determined that strain CDHWZ7 has several excellent biological traits, such as antagonism to pathogenic fungi, nitrogen-fixation ability, cellulose-degradation ability, and IAA-production ability. The genome sequence of strain CDHWZ7 and several biodefense functional genes were also analyzed, revealing the potential use of strain CDHWZ7 in the development of biological agents.

Suggested Citation

  • Xue Yang & Yongli Xie & Youming Qiao & Lan Chen & Tian Wang & Lingling Wu & Junxi Li & Ying Gao, 2023. "Analysis of the Biological Activity and Whole Genome Sequencing of Bacillus cereus CDHWZ7 Isolated from the Rhizosphere of Lycium ruthenicum on the Tibetan Plateau," Agriculture, MDPI, vol. 13(5), pages 1-17, May.
    • Handle: RePEc:gam:jagris:v:13:y:2023:i:5:p:1041-:d:1144579
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2077-0472/13/5/1041/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2077-0472/13/5/1041/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Nicholas Ozede Igiehon & Olubukola Oluranti Babalola, 2018. "Rhizosphere Microbiome Modulators: Contributions of Nitrogen Fixing Bacteria towards Sustainable Agriculture," IJERPH, MDPI, vol. 15(4), pages 1-25, March.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Kainat Hussain & Muhammad Waseem & Iqra Mumtaz & Samreen Riaz, 2022. "Molecular characterization of Deciphering Fungal Community structure in Zea mays L. and Triticum Aestivum L," International Journal of Innovations in Science & Technology, 50sea, vol. 4(3), pages 727-737, June.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jagris:v:13:y:2023:i:5:p:1041-:d:1144579. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.