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Development of SSR Molecular Markers and Genetic Diversity Analysis of TPS Gene Family in Chimonanthus praecox

Author

Listed:
  • Xuemei Fu

    (Yunnan Province Engineering Research Center for Functional Flower Resources and Industrialization, College of Landscape Architecture and Horticulture Sciences, Southwest Forestry University, Kunming 650224, China
    These authors contributed equally to this work.)

  • Nan Yang

    (Yunnan Province Engineering Research Center for Functional Flower Resources and Industrialization, College of Landscape Architecture and Horticulture Sciences, Southwest Forestry University, Kunming 650224, China
    These authors contributed equally to this work.)

  • Yongqin Du

    (Agro-Technical Popularization Service Centre of Jiading District, Shanghai 201800, China)

  • Hafiz Muhammad Kamran

    (Yunnan Province Engineering Research Center for Functional Flower Resources and Industrialization, College of Landscape Architecture and Horticulture Sciences, Southwest Forestry University, Kunming 650224, China)

  • Huabo Wang

    (Yunnan Province Engineering Research Center for Functional Flower Resources and Industrialization, College of Landscape Architecture and Horticulture Sciences, Southwest Forestry University, Kunming 650224, China)

  • Shaoyuan Chen

    (Yunnan Province Engineering Research Center for Functional Flower Resources and Industrialization, College of Landscape Architecture and Horticulture Sciences, Southwest Forestry University, Kunming 650224, China)

  • Longqing Chen

    (Yunnan Province Engineering Research Center for Functional Flower Resources and Industrialization, College of Landscape Architecture and Horticulture Sciences, Southwest Forestry University, Kunming 650224, China)

Abstract

Terpene synthase (TPS) plays a key role in the biosynthesis of terpenoids, which are the most important components of the volatile compounds of wintersweet ( Chimonanthus praecox ). In this study, 52 CpTPS genes were found in wintersweet which were divided into 5 subfamilies. We identified 146 SSRs in the CpTPS genes, and obtained 33 pairs of SSR primers with good polymorphism through amplification in 6 wintersweet samples. Then, these primers were amplified in 69 samples from China’s main wintersweet production areas. Through structural analysis, 69 samples were divided into 2 clusters, and were divided into 4 groups in a genetic cluster analysis, of which SH-33 and SW were separate groups. Through AMOVA analysis, it was found that the variation mainly occurred in the population, and that the gene flow between populations was Nm > 1, so it might lead to population differentiation. In other words, these findings provided useful information for the biosynthesis of terpenoids, the construction of a genetic linkage map, the detection of quantitative trait loci, marker-assisted selection and other aspects of wintersweet.

Suggested Citation

  • Xuemei Fu & Nan Yang & Yongqin Du & Hafiz Muhammad Kamran & Huabo Wang & Shaoyuan Chen & Longqing Chen, 2023. "Development of SSR Molecular Markers and Genetic Diversity Analysis of TPS Gene Family in Chimonanthus praecox," Agriculture, MDPI, vol. 13(4), pages 1-15, April.
    • Handle: RePEc:gam:jagris:v:13:y:2023:i:4:p:893-:d:1126614
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